Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C604
  Reference Plasmid   NZ_CP035861.1
  Reference Plasmid Size   139703
  Reference Plasmid GC Content   0.45
  Reference Plasmid Mobility Type   mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0169322 OFMCCMOF_00082 59043 3 Gut 0.09 protein_coding missense_variant MODERATE 194A>T Glu65Val
M0169323 OFMCCMOF_00047 34095 3 Gut 0.09 protein_coding synonymous_variant LOW 94T>C Leu32Leu
M0169324 OFMCCMOF_00047 34156 3 Gut 0.09 protein_coding missense_variant MODERATE 155A>G Asp52Gly
M0169325 OFMCCMOF_00047 34244 3 Gut 0.09 protein_coding synonymous_variant LOW 243C>T Cys81Cys
M0169326 OFMCCMOF_00047 34364 3 Gut 0.09 protein_coding synonymous_variant LOW 363C>T Arg121Arg
M0169327 OFMCCMOF_00047 34415 3 Gut 0.09 protein_coding synonymous_variant LOW 414T>C Ala138Ala
M0169328 OFMCCMOF_00047 34539 3 Gut 0.09 protein_coding missense_variant MODERATE 538T>A Trp180Arg
M0169329 OFMCCMOF_00047 34554 4 Gut 0.13 protein_coding missense_variant MODERATE 553T>C Trp185Arg
M0169330 OFMCCMOF_00047 34585 4 Gut 0.13 protein_coding missense_variant MODERATE 584T>A Leu195His
M0169331 OFMCCMOF_00039 34634 4 Gut 0.13 protein_coding upstream_gene_variant MODIFIER -4439A>C None
M0169332 OFMCCMOF_00048 34651 3 Gut 0.09 protein_coding missense_variant MODERATE 14C>T Thr5Met
M0169333 OFMCCMOF_00048 34657 3 Gut 0.09 protein_coding missense_variant MODERATE 20A>G Asp7Gly
M0169334 OFMCCMOF_00048 34658 3 Gut 0.09 protein_coding synonymous_variant LOW 21C>T Asp7Asp
M0169335 OFMCCMOF_00048 34663 4 Gut 0.13 protein_coding missense_variant MODERATE 26A>G Lys9Arg
M0169336 OFMCCMOF_00048 34715 3 Gut 0.09 protein_coding synonymous_variant LOW 78C>T Ala26Ala
M0169337 OFMCCMOF_00048 34788 4 Gut 0.13 protein_coding missense_variant MODERATE 151T>G Ser51Ala
M0169338 OFMCCMOF_00048 34906 3 Gut 0.09 protein_coding missense_variant MODERATE 269C>A Thr90Lys
M0169339 OFMCCMOF_00048 35032 3 Gut 0.09 protein_coding missense_variant MODERATE 395G>A Gly132Glu
M0169340 OFMCCMOF_00048 35161 3 Gut 0.09 protein_coding missense_variant MODERATE 524A>G Glu175Gly
M0169341 OFMCCMOF_00040 35199 3 Gut 0.09 protein_coding upstream_gene_variant MODIFIER -3874A>T None
M0169342 OFMCCMOF_00040 35209 3 Gut 0.09 protein_coding upstream_gene_variant MODIFIER -3884G>T None
M0169343 OFMCCMOF_00040 35244 3 Gut 0.09 protein_coding upstream_gene_variant MODIFIER -3919A>G None
M0169344 OFMCCMOF_00040 35280 3 Gut 0.09 protein_coding upstream_gene_variant MODIFIER -3955T>C None
M0169345 OFMCCMOF_00040 35283 3 Gut 0.09 protein_coding upstream_gene_variant MODIFIER -3958A>C None
M0169346 OFMCCMOF_00049 35484 3 Gut 0.09 protein_coding missense_variant MODERATE 82G>A Val28Ile
M0169347 OFMCCMOF_00050 36352 3 Gut 0.09 protein_coding upstream_gene_variant MODIFIER -128A>G None
M0169348 OFMCCMOF_00066 47932 4 Gut 0.13 protein_coding stop_lost&splice_region_variant HIGH 483A>G Ter161Trpext*?
M0169349 OFMCCMOF_00066 48052 4 Gut 0.13 protein_coding synonymous_variant LOW 363C>T Arg121Arg
M0169350 OFMCCMOF_00066 48062 3 Gut 0.09 protein_coding missense_variant MODERATE 353G>A Gly118Glu
M0169351 OFMCCMOF_00066 48103 4 Gut 0.13 protein_coding synonymous_variant LOW 312T>C His104His
M0169352 OFMCCMOF_00066 48166 4 Gut 0.13 protein_coding synonymous_variant LOW 249A>G Lys83Lys
M0169353 OFMCCMOF_00066 48229 4 Gut 0.13 protein_coding missense_variant MODERATE 186G>C Glu62Asp
M0169354 OFMCCMOF_00066 48259 4 Gut 0.13 protein_coding synonymous_variant LOW 156A>C Gly52Gly
M0169355 OFMCCMOF_00066 48343 4 Gut 0.13 protein_coding synonymous_variant LOW 72A>G Thr24Thr
M0169356 OFMCCMOF_00066 48360 4 Gut 0.13 protein_coding missense_variant MODERATE 55A>G Thr19Ala
M0169357 OFMCCMOF_00066 48370 4 Gut 0.13 protein_coding synonymous_variant LOW 45T>C Arg15Arg
M0169358 OFMCCMOF_00067 48397 4 Gut 0.13 protein_coding stop_lost&splice_region_variant HIGH 271T>G Ter91Glyext*?
M0169359 OFMCCMOF_00067 48400 4 Gut 0.13 protein_coding missense_variant MODERATE 268T>G Trp90Gly
M0169360 OFMCCMOF_00067 48416 4 Gut 0.13 protein_coding synonymous_variant LOW 252G>T Ala84Ala
M0169361 OFMCCMOF_00067 48606 4 Gut 0.13 protein_coding missense_variant MODERATE 62T>C Val21Ala
M0169362 OFMCCMOF_00067 48657 4 Gut 0.13 protein_coding missense_variant MODERATE 11A>G Asn4Ser
M0169363 OFMCCMOF_00060 48797 4 Gut 0.13 protein_coding upstream_gene_variant MODIFIER -4951A>T None
M0169364 OFMCCMOF_00068 49260 3 Gut 0.09 protein_coding missense_variant MODERATE 110T>A Val37Asp
M0169365 OFMCCMOF_00069 49396 3 Gut 0.09 protein_coding synonymous_variant LOW 36C>T Thr12Thr
M0169366 OFMCCMOF_00069 49596 3 Gut 0.09 protein_coding missense_variant MODERATE 236G>A Ser79Asn
M0169367 OFMCCMOF_00069 49765 3 Gut 0.09 protein_coding synonymous_variant LOW 405G>A Lys135Lys
M0169368 OFMCCMOF_00069 49800 3 Gut 0.09 protein_coding missense_variant MODERATE 440C>G Ser147Cys
M0169369 OFMCCMOF_00070 50053 3 Gut 0.09 protein_coding missense_variant MODERATE 38G>A Gly13Asp
M0169370 OFMCCMOF_00070 50090 3 Gut 0.09 protein_coding synonymous_variant LOW 75T>C Asp25Asp
M0169371 OFMCCMOF_00070 50092 3 Gut 0.09 protein_coding missense_variant MODERATE 77A>T His26Leu
M0169372 OFMCCMOF_00071 50305 3 Gut 0.09 protein_coding missense_variant MODERATE 22A>G Ser8Gly
M0169373 OFMCCMOF_00063 50656 3 Gut 0.09 protein_coding upstream_gene_variant MODIFIER -4959T>A None
M0169374 OFMCCMOF_00079 55717 6 Gut 0.19 protein_coding missense_variant MODERATE 1219G>C Glu407Gln
M0169375 OFMCCMOF_00079 55751 3 Gut 0.09 protein_coding synonymous_variant LOW 1185T>G Ala395Ala
M0169376 OFMCCMOF_00079 55754 3 Gut 0.09 protein_coding synonymous_variant LOW 1182G>C Ala394Ala
M0169377 OFMCCMOF_00079 55772 3 Gut 0.09 protein_coding synonymous_variant LOW 1164A>G Ala388Ala
M0169378 OFMCCMOF_00079 55818 3 Gut 0.09 protein_coding missense_variant MODERATE 1118T>C Val373Ala
M0169379 OFMCCMOF_00079 55854 3 Gut 0.09 protein_coding missense_variant MODERATE 1082C>T Thr361Ile
M0169380 OFMCCMOF_00167 126949 3 Gut 0.09 protein_coding missense_variant MODERATE 817A>G Met273Val
M0169381 OFMCCMOF_00141 98458 3 Gut 0.09 protein_coding synonymous_variant LOW 1308T>C Gly436Gly
M0169382 OFMCCMOF_00142 99005 3 Gut 0.09 protein_coding synonymous_variant LOW 355C>T Leu119Leu
M0169383 OFMCCMOF_00078 55223 3 Gut 0.09 protein_coding missense_variant MODERATE 448C>G Leu150Val
M0169384 OFMCCMOF_00078 55332 3 Gut 0.09 protein_coding missense_variant MODERATE 557G>A Arg186Lys
M0169385 OFMCCMOF_00079 55471 3 Gut 0.09 protein_coding missense_variant MODERATE 1465G>A Val489Ile
M0169386 OFMCCMOF_00079 55890 3 Gut 0.09 protein_coding missense_variant MODERATE 1046C>T Thr349Ile
M0169387 OFMCCMOF_00079 56575 3 Gut 0.09 protein_coding missense_variant MODERATE 361C>A Pro121Thr
M0169388 OFMCCMOF_00080 57077 3 Gut 0.09 protein_coding missense_variant MODERATE 226G>T Val76Phe
M0169389 OFMCCMOF_00081 57520 3 Gut 0.09 protein_coding missense_variant MODERATE 433C>A Pro145Thr
M0169390 OFMCCMOF_00081 57590 3 Gut 0.09 protein_coding synonymous_variant LOW 363C>T Arg121Arg
M0169391 OFMCCMOF_00081 57641 3 Gut 0.09 protein_coding synonymous_variant LOW 312G>A Arg104Arg
M0169392 OFMCCMOF_00081 57678 3 Gut 0.09 protein_coding missense_variant MODERATE 275T>A Ile92Asn
M0169393 OFMCCMOF_00081 57697 3 Gut 0.09 protein_coding missense_variant MODERATE 256C>A Pro86Thr
M0169394 OFMCCMOF_00081 57704 3 Gut 0.09 protein_coding synonymous_variant LOW 249G>A Val83Val
M0169395 OFMCCMOF_00081 57731 3 Gut 0.09 protein_coding synonymous_variant LOW 222T>G Pro74Pro
M0169396 OFMCCMOF_00081 57737 3 Gut 0.09 protein_coding synonymous_variant LOW 216T>G Leu72Leu
M0169397 OFMCCMOF_00081 57757 3 Gut 0.09 protein_coding missense_variant MODERATE 196A>G Met66Val
M0169398 OFMCCMOF_00081 57770 3 Gut 0.09 protein_coding synonymous_variant LOW 183T>A Gly61Gly
M0169399 OFMCCMOF_00081 57790 3 Gut 0.09 protein_coding missense_variant MODERATE 163G>A Ala55Thr
M0169400 OFMCCMOF_00081 57796 3 Gut 0.09 protein_coding missense_variant MODERATE 157A>G Ile53Val
M0169401 OFMCCMOF_00081 57841 3 Gut 0.09 protein_coding missense_variant MODERATE 112G>A Val38Ile
M0169402 OFMCCMOF_00081 57923 3 Gut 0.09 protein_coding synonymous_variant LOW 30T>C Asp10Asp
M0169403 OFMCCMOF_00081 57933 3 Gut 0.09 protein_coding missense_variant MODERATE 20C>T Ala7Val
M0169404 OFMCCMOF_00081 57938 3 Gut 0.09 protein_coding synonymous_variant LOW 15G>A Lys5Lys
M0169405 OFMCCMOF_00076 57964 3 Gut 0.09 protein_coding upstream_gene_variant MODIFIER -4484A>G None
M0169406 OFMCCMOF_00076 58070 3 Gut 0.09 protein_coding upstream_gene_variant MODIFIER -4590C>A None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
OFMCCMOF_00133 VFG034574 Dispersin 94.8 3.1e-60 1 116 1.0 1 Others dispersin experiment
OFMCCMOF_00037 VFG035924 Dispersin 82.2 1.2e-93 1 208 1.0 1 Others ATP-binding protein AatC prediction
OFMCCMOF_00038 VFG035918 Dispersin 83.1 1.5e-119 1 255 1.0 1 Others AatB prediction
OFMCCMOF_00039 VFG035911 Dispersin 80.5 1.1e-177 1 400 1.0 1 Others outer membrane protein AatA prediction
OFMCCMOF_00040 VFG035904 Dispersin 82.9 2.3e-161 6 377 0.9867 0.9841 Others permease AatP prediction
OFMCCMOF_00133 VFG034574 Dispersin 94.8 2.3e-59 1 116 1.0 1 Others dispersin prediction
OFMCCMOF_00141 VFG035359 AAI/SCI-II T6SS 80.1 7.6e-237 1 492 1.0 1 Effector delivery system type VI secretion system contractile sheath large subunit prediction
OFMCCMOF_00148 VFG035394 AAI/SCI-II T6SS 72.7 1.7e-39 1 99 1.0 1 Effector delivery system PAAR domain-containing protein prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
OFMCCMOF_00095 AWU48011.1|GH23 98.2 1.66e-122 1 169 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
OFMCCMOF_00124 1.E.53.1.11 97.1 2.9e-32 36 103 0.6602 1.0000 1 Channels/Pores 1.E Holins 1.E.53 The Toxic Hok/Gef Protein (Hok/Gef) Family