Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C607
  Reference Plasmid   NZ_CP035868.1
  Reference Plasmid Size   90662
  Reference Plasmid GC Content   0.49
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0169456 KKFBMGLM_00024 19287 4 Gut 0.44 protein_coding missense_variant MODERATE 521A>G Asp174Gly
M0169457 KKFBMGLM_00022 17700 3 Gut 0.33 protein_coding synonymous_variant LOW 252T>C Asp84Asp
M0169458 KKFBMGLM_00103 80633 3 Gut 0.33 protein_coding missense_variant MODERATE 285A>T Glu95Asp
M0169459 KKFBMGLM_00103 80720 3 Gut 0.33 protein_coding synonymous_variant LOW 372T>C Ala124Ala






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
KKFBMGLM_00019 VFG002039 Heat-stable toxin (ST) 98.6 9.6e-36 1 72 1.0 1 Exotoxin heat stable enterotoxin I experiment
KKFBMGLM_00024 VFG036066 Heat-labile toxin (LT) 99.1 4.5e-129 1 218 1.0 0.845 Exotoxin heat-labile enterotoxin A prepeptide (from human) experiment
KKFBMGLM_00025 VFG036068 Heat-labile toxin (LT) 100 4.8e-67 1 124 1.0 1 Exotoxin enterotoxin subunit B (from human) experiment
KKFBMGLM_00019 VFG002039 Heat-stable toxin (ST) 98.6 7.2e-35 1 72 1.0 1 Exotoxin heat stable enterotoxin I prediction
KKFBMGLM_00024 VFG036065 Heat-labile toxin (LT) 99.5 8.9e-129 1 218 1.0 0.845 Exotoxin heat-labile enterotoxin A prepeptide (from human) prediction
KKFBMGLM_00025 VFG036068 Heat-labile toxin (LT) 100 3.6e-66 1 124 1.0 1 Exotoxin enterotoxin subunit B (from human) prediction
KKFBMGLM_00032 VFG001434 Adhesive fimbriae 93.4 8.9e-96 1 196 1.0 1 Adherence CS12 fimbria major subunit protein precursor prediction
KKFBMGLM_00033 VFG042487 Adhesive fimbriae 99.1 9.2e-132 1 233 1.0 0.951 Adherence CS12 fimbria chaperone protein prediction
KKFBMGLM_00034 VFG042488 Adhesive fimbriae 96.2 9.3e-51 17 122 0.8689 1 Adherence CS12 fimbria chaperone protein prediction
KKFBMGLM_00035 VFG042489 Adhesive fimbriae 99.3 0 1 835 1.0 1 Adherence CS12 fimbria outer membrane usher protein precursor prediction
KKFBMGLM_00036 VFG042490 Adhesive fimbriae 100 6.6e-74 2 135 0.9926 1 Adherence CS12 fimbria chaperone protein precursor prediction
KKFBMGLM_00037 VFG042491 Adhesive fimbriae 100 1.8e-95 1 177 1.0 1 Adherence CS12 fimbria minor subunit protein precursor prediction
KKFBMGLM_00038 VFG042492 Adhesive fimbriae 99.5 6.2e-248 1 416 1.0 1 Adherence CS12 fimbria putative adhesin protein prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
KKFBMGLM_00024 PHI:698 CtxA 80.7 3.7e-106 1 218 1.0000 0.8450 primates cholera Cholera toxin (CT) reduced virulence
KKFBMGLM_00025 PHI:699 CtxB 78.2 1.9e-51 1 124 1.0000 1.0000 primates cholera Cholera toxin (CT) reduced virulence
KKFBMGLM_00055 PHI:3113 stbA 97.9 2.1e-182 1 326 1.0000 1.0000 rodents neonatal meningitis stability protein for the plasmid reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
KKFBMGLM_00068 QAY42704.1|GH23 100 7.04e-124 1 169 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
KKFBMGLM_00024 1.C.72.4.1 99.1 2.7e-128 1 218 1.0000 0.8450 1 Channels/Pores 1.C Pore-Forming Toxins (Proteins and Peptides) 1.C.72 The Pertussis Toxin (PTX) Family