Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C624
  Reference Plasmid   NZ_CP038257.1
  Reference Plasmid Size   127669
  Reference Plasmid GC Content   0.68
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0095654 HAFHHKCE_00109 97539 3 Skin 0.11 protein_coding missense_variant MODERATE 691C>G Leu231Val
M0095655 HAFHHKCE_00012 7492 12 Skin 0.44 protein_coding missense_variant MODERATE 1687T>G Ser563Ala
M0095656 HAFHHKCE_00009 4660 5 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -13A>G None
M0095657 HAFHHKCE_00009 4666 6 Skin 0.22 protein_coding upstream_gene_variant MODIFIER -19G>A None
M0095658 HAFHHKCE_00010 4716 7 Skin 0.26 protein_coding synonymous_variant LOW 15T>C Arg5Arg
M0095659 HAFHHKCE_00012 8502 11 Skin 0.41 protein_coding missense_variant MODERATE 677C>T Ala226Val
M0095660 HAFHHKCE_00017 14415 11 Skin 0.41 protein_coding synonymous_variant LOW 855T>C Ala285Ala
M0095661 HAFHHKCE_00017 14627 8 Skin 0.30 protein_coding missense_variant MODERATE 643C>G Leu215Val
M0095662 HAFHHKCE_00017 14886 13 Skin 0.48 protein_coding synonymous_variant LOW 384T>C His128His
M0095663 HAFHHKCE_00017 14931 13 Skin 0.48 protein_coding synonymous_variant LOW 339T>C Ala113Ala
M0095664 HAFHHKCE_00017 14976 13 Skin 0.48 protein_coding synonymous_variant LOW 294T>G Pro98Pro
M0095665 HAFHHKCE_00018 15596 12 Skin 0.44 protein_coding synonymous_variant LOW 1359T>C Asp453Asp
M0095666 HAFHHKCE_00018 15599 12 Skin 0.44 protein_coding synonymous_variant LOW 1356T>C Val452Val
M0095667 HAFHHKCE_00018 15602 12 Skin 0.44 protein_coding synonymous_variant LOW 1353T>C Thr451Thr
M0095668 HAFHHKCE_00018 15647 12 Skin 0.44 protein_coding synonymous_variant LOW 1308T>C Gly436Gly
M0095669 HAFHHKCE_00018 15650 12 Skin 0.44 protein_coding synonymous_variant LOW 1305T>C Thr435Thr
M0095670 HAFHHKCE_00018 15662 12 Skin 0.44 protein_coding synonymous_variant LOW 1293T>C Leu431Leu
M0095671 HAFHHKCE_00018 15698 12 Skin 0.44 protein_coding synonymous_variant LOW 1257C>T Thr419Thr
M0095672 HAFHHKCE_00018 15740 12 Skin 0.44 protein_coding synonymous_variant LOW 1215C>T Thr405Thr
M0095673 HAFHHKCE_00018 15743 12 Skin 0.44 protein_coding missense_variant MODERATE 1212C>G Ile404Met
M0095674 HAFHHKCE_00018 15745 12 Skin 0.44 protein_coding missense_variant MODERATE 1210A>C Ile404Leu
M0095675 HAFHHKCE_00018 15752 12 Skin 0.44 protein_coding synonymous_variant LOW 1203C>T Asn401Asn
M0095676 HAFHHKCE_00018 15830 12 Skin 0.44 protein_coding synonymous_variant LOW 1125A>G Gly375Gly
M0095677 HAFHHKCE_00018 15839 12 Skin 0.44 protein_coding synonymous_variant LOW 1116G>A Glu372Glu
M0095678 HAFHHKCE_00018 15842 12 Skin 0.44 protein_coding synonymous_variant LOW 1113T>C Pro371Pro
M0095679 HAFHHKCE_00018 15859 12 Skin 0.44 protein_coding missense_variant MODERATE 1096G>A Val366Ile
M0095680 HAFHHKCE_00018 15869 12 Skin 0.44 protein_coding synonymous_variant LOW 1086A>C Gly362Gly
M0095681 HAFHHKCE_00018 15914 12 Skin 0.44 protein_coding synonymous_variant LOW 1041A>G Glu347Glu
M0095682 HAFHHKCE_00018 15926 12 Skin 0.44 protein_coding synonymous_variant LOW 1029C>G Val343Val
M0095683 HAFHHKCE_00018 15935 12 Skin 0.44 protein_coding synonymous_variant LOW 1020A>C Ala340Ala
M0095684 HAFHHKCE_00018 15974 12 Skin 0.44 protein_coding synonymous_variant LOW 981A>C Gly327Gly
M0095685 HAFHHKCE_00018 15989 12 Skin 0.44 protein_coding synonymous_variant LOW 966G>C Arg322Arg
M0095686 HAFHHKCE_00018 15992 12 Skin 0.44 protein_coding synonymous_variant LOW 963C>T Asp321Asp
M0095687 HAFHHKCE_00018 15998 12 Skin 0.44 protein_coding missense_variant MODERATE 957A>C Glu319Asp
M0095688 HAFHHKCE_00018 16013 12 Skin 0.44 protein_coding synonymous_variant LOW 942T>C Tyr314Tyr
M0095689 HAFHHKCE_00018 16040 12 Skin 0.44 protein_coding synonymous_variant LOW 915C>T Asp305Asp
M0095690 HAFHHKCE_00018 16055 12 Skin 0.44 protein_coding synonymous_variant LOW 900G>A Gln300Gln
M0095691 HAFHHKCE_00018 16061 12 Skin 0.44 protein_coding synonymous_variant LOW 894T>C Ile298Ile
M0095692 HAFHHKCE_00018 16069 12 Skin 0.44 protein_coding missense_variant MODERATE 886G>A Val296Ile
M0095693 HAFHHKCE_00018 16085 12 Skin 0.44 protein_coding synonymous_variant LOW 870C>A Gly290Gly
M0095694 HAFHHKCE_00018 16088 12 Skin 0.44 protein_coding synonymous_variant LOW 867G>C Ala289Ala
M0095695 HAFHHKCE_00018 16103 12 Skin 0.44 protein_coding synonymous_variant LOW 852T>C Pro284Pro
M0095696 HAFHHKCE_00018 16124 12 Skin 0.44 protein_coding synonymous_variant LOW 831T>C Ser277Ser
M0095697 HAFHHKCE_00018 16139 12 Skin 0.44 protein_coding synonymous_variant LOW 816T>C Asp272Asp
M0095698 HAFHHKCE_00018 16166 12 Skin 0.44 protein_coding synonymous_variant LOW 789G>C Gly263Gly
M0095699 HAFHHKCE_00018 16169 12 Skin 0.44 protein_coding synonymous_variant LOW 786C>G Thr262Thr
M0095700 HAFHHKCE_00018 16184 12 Skin 0.44 protein_coding synonymous_variant LOW 771G>T Arg257Arg
M0095701 HAFHHKCE_00018 16195 12 Skin 0.44 protein_coding missense_variant MODERATE 760G>A Asp254Asn
M0095702 HAFHHKCE_00018 16205 12 Skin 0.44 protein_coding synonymous_variant LOW 750T>C Tyr250Tyr
M0095703 HAFHHKCE_00018 16226 12 Skin 0.44 protein_coding synonymous_variant LOW 729T>C Phe243Phe
M0095704 HAFHHKCE_00018 16521 12 Skin 0.44 protein_coding missense_variant MODERATE 434T>C Val145Ala
M0095705 HAFHHKCE_00018 16532 12 Skin 0.44 protein_coding synonymous_variant LOW 423G>T Pro141Pro
M0095706 HAFHHKCE_00018 16685 12 Skin 0.44 protein_coding synonymous_variant LOW 270C>T Pro90Pro
M0095707 HAFHHKCE_00018 16926 13 Skin 0.48 protein_coding missense_variant MODERATE 29C>T Thr10Ile
M0095708 HAFHHKCE_00019 16986 13 Skin 0.48 protein_coding synonymous_variant LOW 717G>A Ala239Ala
M0095709 HAFHHKCE_00019 17358 13 Skin 0.48 protein_coding synonymous_variant LOW 345G>C Leu115Leu
M0095710 HAFHHKCE_00020 17864 13 Skin 0.48 protein_coding synonymous_variant LOW 435G>A Gly145Gly
M0095711 HAFHHKCE_00020 17909 13 Skin 0.48 protein_coding missense_variant MODERATE 390A>T Arg130Ser
M0095712 HAFHHKCE_00020 17911 13 Skin 0.48 protein_coding synonymous_variant LOW 388A>C Arg130Arg
M0095713 HAFHHKCE_00020 17912 13 Skin 0.48 protein_coding synonymous_variant LOW 387G>C Ala129Ala
M0095714 HAFHHKCE_00020 17923 13 Skin 0.48 protein_coding synonymous_variant LOW 376T>C Leu126Leu
M0095715 HAFHHKCE_00020 17966 13 Skin 0.48 protein_coding synonymous_variant LOW 333G>C Val111Val
M0095716 HAFHHKCE_00020 17969 13 Skin 0.48 protein_coding synonymous_variant LOW 330T>C Gly110Gly
M0095717 HAFHHKCE_00020 18071 12 Skin 0.44 protein_coding synonymous_variant LOW 228G>C Arg76Arg
M0095718 HAFHHKCE_00020 18195 11 Skin 0.41 protein_coding missense_variant MODERATE 104A>G Asn35Ser
M0095719 HAFHHKCE_00020 18197 11 Skin 0.41 protein_coding synonymous_variant LOW 102G>A Gly34Gly
M0095720 HAFHHKCE_00020 18271 11 Skin 0.41 protein_coding missense_variant MODERATE 28G>A Val10Met
M0095721 HAFHHKCE_00021 18334 11 Skin 0.41 protein_coding synonymous_variant LOW 297T>C Phe99Phe
M0095722 HAFHHKCE_00021 18343 11 Skin 0.41 protein_coding synonymous_variant LOW 288A>G Ala96Ala
M0095723 HAFHHKCE_00021 18346 11 Skin 0.41 protein_coding synonymous_variant LOW 285G>C Ala95Ala
M0095724 HAFHHKCE_00021 18415 11 Skin 0.41 protein_coding synonymous_variant LOW 216G>C Ala72Ala
M0095725 HAFHHKCE_00025 20980 9 Skin 0.33 protein_coding missense_variant MODERATE 295C>G Leu99Val
M0095726 HAFHHKCE_00026 21367 3 Skin 0.11 protein_coding synonymous_variant LOW 576G>A Thr192Thr
M0095727 HAFHHKCE_00026 21379 3 Skin 0.11 protein_coding synonymous_variant LOW 564G>C Gly188Gly
M0095728 HAFHHKCE_00026 21403 3 Skin 0.11 protein_coding synonymous_variant LOW 540T>C Asp180Asp
M0095729 HAFHHKCE_00026 21423 3 Skin 0.11 protein_coding missense_variant MODERATE 520G>A Ala174Thr
M0095730 HAFHHKCE_00026 21427 3 Skin 0.11 protein_coding synonymous_variant LOW 516T>C Ala172Ala
M0095731 HAFHHKCE_00016 13539 3 Skin 0.11 protein_coding synonymous_variant LOW 786C>T Gly262Gly
M0095732 HAFHHKCE_00016 14286 4 Skin 0.15 protein_coding synonymous_variant LOW 39G>A Ala13Ala






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group
HAFHHKCE_00030 Cobalt (Co), Nickel (Ni) 91.1 9.1e-49 1 101 1.0000 0.9099 prediction
MNACKCKM_00030 Cobalt (Co), Nickel (Ni) 91.1 9.1e-49 1 101 1.0000 0.9099 prediction






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
HAFHHKCE_00050 QBR75597.1|GH184 100 2.26e-303 1 421 1 1
HAFHHKCE_00071 QBR75615.1|CBM50 100 0 1 1057 1 1
MNACKCKM_00050 QBR75597.1|GH184 100 2.26e-303 1 421 1 1
MNACKCKM_00071 QBR75615.1|CBM50 100 0 1 1057 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term