Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C672
  Reference Plasmid   NZ_CP045917.1
  Reference Plasmid Size   468631
  Reference Plasmid GC Content   0.57
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0097195 KMEIDAKP_00221 213152 13 Skin 0.14 protein_coding upstream_gene_variant MODIFIER -2367C>T None
M0097196 KMEIDAKP_00449 373787 9 Skin 0.10 protein_coding upstream_gene_variant MODIFIER -4911C>T None
M0097197 KMEIDAKP_00463 384566 7 Skin 0.08 protein_coding synonymous_variant LOW 90T>C Ser30Ser
M0097198 KMEIDAKP_00463 384571 7 Skin 0.08 protein_coding missense_variant MODERATE 95G>C Arg32Pro
M0097199 KMEIDAKP_00463 384591 7 Skin 0.08 protein_coding missense_variant MODERATE 115A>C Asn39His
M0097200 KMEIDAKP_00465 386666 8 Skin 0.09 protein_coding missense_variant MODERATE 238A>G Thr80Ala
M0097201 KMEIDAKP_00223 212222 6 Skin 0.07 protein_coding missense_variant MODERATE 242C>T Ala81Val
M0097202 KMEIDAKP_00229 216439 4 Skin 0.04 protein_coding synonymous_variant LOW 1809T>C Gly603Gly
M0097203 KMEIDAKP_00229 216118 5 Skin 0.06 protein_coding synonymous_variant LOW 1488C>T Asp496Asp
M0097204 KMEIDAKP_00229 216242 3 Skin 0.03 protein_coding missense_variant MODERATE 1612G>A Ala538Thr
M0097205 KMEIDAKP_00451 372160 4 Skin 0.04 protein_coding synonymous_variant LOW 1278G>A Lys426Lys
M0097206 KMEIDAKP_00451 372175 4 Skin 0.04 protein_coding synonymous_variant LOW 1263A>G Ala421Ala
M0097207 KMEIDAKP_00451 372190 4 Skin 0.04 protein_coding synonymous_variant LOW 1248T>A Ala416Ala
M0097208 KMEIDAKP_00451 372211 4 Skin 0.04 protein_coding synonymous_variant LOW 1227T>C Gly409Gly
M0097209 KMEIDAKP_00451 372214 4 Skin 0.04 protein_coding synonymous_variant LOW 1224C>T Thr408Thr
M0097210 KMEIDAKP_00451 372243 4 Skin 0.04 protein_coding missense_variant MODERATE 1195G>T Ala399Ser
M0097211 KMEIDAKP_00451 372304 4 Skin 0.04 protein_coding synonymous_variant LOW 1134T>C Ala378Ala
M0097212 KMEIDAKP_00451 372310 4 Skin 0.04 protein_coding synonymous_variant LOW 1128A>G Leu376Leu
M0097213 KMEIDAKP_00451 372322 4 Skin 0.04 protein_coding synonymous_variant LOW 1116A>G Leu372Leu
M0097214 KMEIDAKP_00451 372337 4 Skin 0.04 protein_coding synonymous_variant LOW 1101G>T Leu367Leu
M0097215 KMEIDAKP_00451 372364 4 Skin 0.04 protein_coding synonymous_variant LOW 1074G>T Val358Val
M0097216 KMEIDAKP_00451 372400 4 Skin 0.04 protein_coding synonymous_variant LOW 1038T>C His346His
M0097217 KMEIDAKP_00451 372481 3 Skin 0.03 protein_coding synonymous_variant LOW 957A>G Pro319Pro
M0097218 KMEIDAKP_00451 372511 3 Skin 0.03 protein_coding synonymous_variant LOW 927T>G Val309Val
M0097219 KMEIDAKP_00451 372529 3 Skin 0.03 protein_coding synonymous_variant LOW 909T>C Phe303Phe
M0097220 KMEIDAKP_00451 372535 3 Skin 0.03 protein_coding synonymous_variant LOW 903C>T Gly301Gly
M0097221 KMEIDAKP_00451 372544 3 Skin 0.03 protein_coding synonymous_variant LOW 894T>G Leu298Leu
M0097222 KMEIDAKP_00451 372547 3 Skin 0.03 protein_coding synonymous_variant LOW 891T>C Ser297Ser
M0097223 KMEIDAKP_00451 372550 3 Skin 0.03 protein_coding synonymous_variant LOW 888G>C Ser296Ser
M0097224 KMEIDAKP_00451 372556 3 Skin 0.03 protein_coding synonymous_variant LOW 882C>T Arg294Arg
M0097225 KMEIDAKP_00451 372583 3 Skin 0.03 protein_coding synonymous_variant LOW 855T>C Ile285Ile
M0097226 KMEIDAKP_00451 372613 3 Skin 0.03 protein_coding synonymous_variant LOW 825T>C Ala275Ala
M0097227 KMEIDAKP_00451 373018 5 Skin 0.06 protein_coding synonymous_variant LOW 420T>C Thr140Thr
M0097228 KMEIDAKP_00451 373069 3 Skin 0.03 protein_coding synonymous_variant LOW 369T>C Phe123Phe
M0097229 KMEIDAKP_00451 373072 3 Skin 0.03 protein_coding synonymous_variant LOW 366T>C Gly122Gly
M0097230 KMEIDAKP_00451 373093 3 Skin 0.03 protein_coding synonymous_variant LOW 345C>T Val115Val
M0097231 KMEIDAKP_00451 373111 3 Skin 0.03 protein_coding synonymous_variant LOW 327A>C Gly109Gly
M0097232 KMEIDAKP_00451 373133 3 Skin 0.03 protein_coding missense_variant MODERATE 305C>G Ala102Gly
M0097233 KMEIDAKP_00451 373141 3 Skin 0.03 protein_coding synonymous_variant LOW 297A>G Leu99Leu
M0097234 KMEIDAKP_00451 373143 3 Skin 0.03 protein_coding synonymous_variant LOW 295C>T Leu99Leu
M0097235 KMEIDAKP_00451 373153 3 Skin 0.03 protein_coding synonymous_variant LOW 285A>G Leu95Leu
M0097236 KMEIDAKP_00450 370104 3 Skin 0.03 protein_coding missense_variant MODERATE 1103T>C Ile368Thr
M0097237 KMEIDAKP_00450 370176 3 Skin 0.03 protein_coding missense_variant MODERATE 1175T>A Phe392Tyr
M0097238 KMEIDAKP_00450 370178 3 Skin 0.03 protein_coding missense_variant MODERATE 1177A>G Thr393Ala
M0097239 KMEIDAKP_00450 371165 4 Skin 0.04 protein_coding missense_variant MODERATE 2164G>C Ala722Pro
M0097240 KMEIDAKP_00450 370606 3 Skin 0.03 protein_coding synonymous_variant LOW 1605G>A Glu535Glu
M0097241 KMEIDAKP_00451 372682 3 Skin 0.03 protein_coding synonymous_variant LOW 756T>C Ser252Ser
M0097242 KMEIDAKP_00451 372685 3 Skin 0.03 protein_coding synonymous_variant LOW 753G>C Pro251Pro
M0097243 KMEIDAKP_00451 372712 3 Skin 0.03 protein_coding synonymous_variant LOW 726C>T Thr242Thr
M0097244 KMEIDAKP_00451 372735 3 Skin 0.03 protein_coding synonymous_variant LOW 703T>C Leu235Leu
M0097245 KMEIDAKP_00451 372748 3 Skin 0.03 protein_coding synonymous_variant LOW 690T>C Ala230Ala
M0097246 KMEIDAKP_00451 372754 3 Skin 0.03 protein_coding synonymous_variant LOW 684A>G Ala228Ala
M0097247 KMEIDAKP_00451 372763 3 Skin 0.03 protein_coding synonymous_variant LOW 675T>C Tyr225Tyr
M0097248 KMEIDAKP_00451 372769 3 Skin 0.03 protein_coding synonymous_variant LOW 669T>C Phe223Phe
M0097249 KMEIDAKP_00451 372783 3 Skin 0.03 protein_coding synonymous_variant LOW 655C>T Leu219Leu
M0097250 KMEIDAKP_00451 372793 3 Skin 0.03 protein_coding synonymous_variant LOW 645A>G Thr215Thr
M0097251 KMEIDAKP_00451 372805 3 Skin 0.03 protein_coding synonymous_variant LOW 633T>C Asp211Asp
M0097252 KMEIDAKP_00451 372817 3 Skin 0.03 protein_coding synonymous_variant LOW 621C>T Phe207Phe
M0097253 KMEIDAKP_00451 372823 3 Skin 0.03 protein_coding synonymous_variant LOW 615G>A Pro205Pro
M0097254 KMEIDAKP_00451 372826 3 Skin 0.03 protein_coding synonymous_variant LOW 612A>G Leu204Leu
M0097255 KMEIDAKP_00451 372844 3 Skin 0.03 protein_coding synonymous_variant LOW 594T>G Gly198Gly
M0097256 KMEIDAKP_00451 372874 3 Skin 0.03 protein_coding synonymous_variant LOW 564T>G Gly188Gly
M0097257 KMEIDAKP_00451 372892 3 Skin 0.03 protein_coding synonymous_variant LOW 546C>A Ala182Ala
M0097258 KMEIDAKP_00451 372919 3 Skin 0.03 protein_coding synonymous_variant LOW 519G>A Pro173Pro
M0097259 KMEIDAKP_00451 372944 3 Skin 0.03 protein_coding missense_variant MODERATE 494A>G Lys165Arg
M0097260 KMEIDAKP_00451 372951 3 Skin 0.03 protein_coding missense_variant MODERATE 487G>A Val163Ile
M0097261 KMEIDAKP_00451 372958 3 Skin 0.03 protein_coding synonymous_variant LOW 480C>T Phe160Phe
M0097262 KMEIDAKP_00451 372961 3 Skin 0.03 protein_coding synonymous_variant LOW 477G>A Leu159Leu
M0097263 KMEIDAKP_00451 372964 3 Skin 0.03 protein_coding synonymous_variant LOW 474T>C Tyr158Tyr
M0097264 KMEIDAKP_00448 368959 4 Skin 0.04 protein_coding upstream_gene_variant MODIFIER -763A>G None
M0097265 KMEIDAKP_00450 369563 3 Skin 0.03 protein_coding missense_variant MODERATE 562C>T Pro188Ser
M0097266 KMEIDAKP_00450 370251 3 Skin 0.03 protein_coding missense_variant MODERATE 1250T>C Val417Ala
M0097267 KMEIDAKP_00224 212691 3 Skin 0.03 protein_coding synonymous_variant LOW 96T>C Thr32Thr






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group
KMEIDAKP_00141 Tellurium (Te) 71.3 2.9e-138 1 340 0.9942 0.9884 experiment
KMEIDAKP_00142 Tellurium (Te) 70.3 3.6e-77 1 192 1.0000 1.0000 experiment
KMEIDAKP_00143 Tellurium (Te) 70.7 5.6e-78 1 191 0.9948 1.0000 experiment
KMEIDAKP_00141 Tellurium (Te) 72.2 5e-139 1 340 0.9942 0.9884 prediction
KMEIDAKP_00142 Tellurium (Te) 72.4 1e-77 1 192 1.0000 1.0000 prediction
KMEIDAKP_00143 Tellurium (Te) 71.2 5.7e-76 1 191 0.9948 1.0000 prediction






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
KMEIDAKP_00443 ARO:3002660 99.2 3.57e-180 1 247 1.0000 0.8885 aminoglycoside antibiotic APH(6) antibiotic inactivation
KMEIDAKP_00448 ARO:3002639 100 3.6e-203 1 267 1.0000 1.0000 aminoglycoside antibiotic APH(3'') antibiotic inactivation
KMEIDAKP_00455 ARO:3000410 100 2e-198 6 283 0.9823 0.9964 sulfonamide antibiotic sulfonamide resistant sul antibiotic target replacement
KMEIDAKP_00459 ARO:3000174 94.6 2.91e-260 1 391 1.0000 1.0000 tetracycline antibiotic major facilitator superfamily (MFS) antibiotic efflux pump antibiotic efflux
KMEIDAKP_00461 ARO:3005043 100 4.13e-278 1 404 1.0000 1.0000 phenicol antibiotic major facilitator superfamily (MFS) antibiotic efflux pump antibiotic efflux
KMEIDAKP_00462 ARO:3002578 99.5 2.85e-154 1 210 1.0000 0.8046 aminoglycoside antibiotic AAC(6') antibiotic inactivation






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
KMEIDAKP_00035 PHI:10380 RecA 72.9 2.4e-139 1 328 0.9507 0.9398 nematodes nosocomial infection DNA strand exchange and recombination protein reduced virulence
KMEIDAKP_00221 PHI:123265 aigC 73.8 0 1 794 1.0000 0.9987 eudicots infection acyl-homoserine-lactone acylase reduced virulence
KMEIDAKP_00463 PHI:9804 int 100 3e-14 1 37 0.1098 1.0000 rodents gastroenteritis integrase reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
KMEIDAKP_00281 QGQ00737.1|GH23 100 1.29e-167 1 234 1 1
KMEIDAKP_00518 UUC21733.1|GT2 97.1 3.17e-192 1 273 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
KMEIDAKP_00141 2.A.109.1.1 71.9 1.8e-139 1 340 0.9884 0.9884 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.109 The Tellurium Ion Resistance (TerC) Family
KMEIDAKP_00208 2.A.53.3.1 74.7 2.3e-204 1 494 0.9980 0.9939 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.53 The Sulfate Permease (SulP) Family
KMEIDAKP_00451 2.A.53.3.1 74.5 1e-204 1 494 0.9980 0.9939 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.53 The Sulfate Permease (SulP) Family
KMEIDAKP_00461 2.A.1.2.110 92.8 4e-202 1 404 1.0000 1.0000 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.1 The Major Facilitator Superfamily (MFS)