Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C771
  Reference Plasmid   NZ_CP059841.1
  Reference Plasmid Size   96603
  Reference Plasmid GC Content   0.48
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0177293 ELFCHKLA_00086 76320 4 Gut 0.22 protein_coding upstream_gene_variant MODIFIER -3978A>G None
M0177294 ELFCHKLA_00092 76861 4 Gut 0.22 protein_coding synonymous_variant LOW 141C>T Asp47Asp
M0177295 ELFCHKLA_00092 76965 4 Gut 0.22 protein_coding missense_variant MODERATE 245A>G Lys82Arg
M0177296 ELFCHKLA_00093 77623 4 Gut 0.22 protein_coding missense_variant MODERATE 127G>A Val43Ile
M0177297 ELFCHKLA_00093 78242 4 Gut 0.22 protein_coding upstream_gene_variant MODIFIER -493A>T None
M0177298 ELFCHKLA_00095 79860 4 Gut 0.22 protein_coding synonymous_variant LOW 939C>T Ile313Ile
M0177299 ELFCHKLA_00004 1805 3 Gut 0.17 protein_coding synonymous_variant LOW 24A>T Ala8Ala
M0177300 ELFCHKLA_00082 73116 3 Gut 0.17 protein_coding upstream_gene_variant MODIFIER -3839A>T None
M0177301 ELFCHKLA_00098 82187 3 Gut 0.17 protein_coding synonymous_variant LOW 267A>T Ala89Ala






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
ELFCHKLA_00001 VFG000764 Per 100 5.1e-72 1 129 1.0 1 Regulation BfpV experiment
ELFCHKLA_00002 VFG000765 Per 100 9.7e-46 1 89 1.0 1 Regulation BfpW experiment
ELFCHKLA_00060 VFG001445 TraJ 98.5 1.2e-113 1 200 0.8772 0.995 Invasion unknown protein experiment
ELFCHKLA_00097 VFG042784 BFP 100 3.5e-101 1 193 1.0 1 Adherence major pilin structural unit bundlin experiment
ELFCHKLA_00098 VFG000750 BFP 100 9.6e-74 1 133 1.0 1 Adherence BFP biogenesis protein BfpG experiment
ELFCHKLA_00099 VFG000751 BFP 100 5.1e-302 1 552 1.0 1 Adherence BfpB secretin experiment
ELFCHKLA_00100 VFG000752 BFP 100 2.5e-242 1 402 1.0 1 Adherence BfpC, bitopic inner membrane protein experiment
ELFCHKLA_00101 VFG042788 BFP 100 1.4e-85 1 164 1.0 1 Adherence periplasmic protein experiment
ELFCHKLA_00102 VFG042789 BFP 100 3.40000000000022e-311 1 534 1.0 0.9981 Adherence assembly ATPase experiment
ELFCHKLA_00103 VFG000755 BFP 100 3.3e-198 1 352 1.0 1 Adherence BfpE, polytopic inner membrane protein experiment
ELFCHKLA_00104 VFG042791 BFP 100 2.5e-187 1 331 1.0 1 Adherence retraction ATPase experiment
ELFCHKLA_00105 VFG000757 BFP 100 2.3e-145 1 249 1.0 1 Adherence prepilin peptidase BfpP experiment
ELFCHKLA_00106 VFG042793 BFP 100 5.7e-83 1 148 1.0 1 Adherence lipoprotein experiment
ELFCHKLA_00107 VFG042794 BFP 100 1e-97 1 181 1.0 1 Adherence minor pilin subunit BfpI experiment
ELFCHKLA_00108 VFG000760 BFP 100 8.8e-102 1 183 1.0 1 Adherence minor pilin subunit BfpJ experiment
ELFCHKLA_00109 VFG042796 BFP 100 2.4e-87 1 162 1.0 1 Adherence minor pilin subunit BfpK experiment
ELFCHKLA_00110 VFG000762 BFP 100 1.5e-71 1 130 1.0 0.8725 Adherence BFP biogenesis protein BfpL experiment
ELFCHKLA_00001 VFG000764 Per 100 3.8e-71 1 129 1.0 1 Regulation BfpV prediction
ELFCHKLA_00002 VFG000765 Per 100 7.2e-45 1 89 1.0 1 Regulation BfpW prediction
ELFCHKLA_00060 VFG001445 TraJ 98.5 9.3e-113 1 200 0.8772 0.995 Invasion unknown protein prediction
ELFCHKLA_00097 VFG042784 BFP 100 2.6e-100 1 193 1.0 1 Adherence major pilin structural unit bundlin prediction
ELFCHKLA_00098 VFG000750 BFP 100 7.2e-73 1 133 1.0 1 Adherence BFP biogenesis protein BfpG prediction
ELFCHKLA_00099 VFG000751 BFP 100 3.8e-301 1 552 1.0 1 Adherence BfpB secretin prediction
ELFCHKLA_00100 VFG000752 BFP 100 1.9e-241 1 402 1.0 1 Adherence BfpC, bitopic inner membrane protein prediction
ELFCHKLA_00101 VFG042788 BFP 100 1e-84 1 164 1.0 1 Adherence periplasmic protein prediction
ELFCHKLA_00102 VFG042789 BFP 100 2.50000000000002e-310 1 534 1.0 0.9981 Adherence assembly ATPase prediction
ELFCHKLA_00103 VFG000755 BFP 100 2.5e-197 1 352 1.0 1 Adherence BfpE, polytopic inner membrane protein prediction
ELFCHKLA_00104 VFG042791 BFP 100 1.9e-186 1 331 1.0 1 Adherence retraction ATPase prediction
ELFCHKLA_00105 VFG000757 BFP 100 1.7e-144 1 249 1.0 1 Adherence prepilin peptidase BfpP prediction
ELFCHKLA_00106 VFG042793 BFP 100 4.2e-82 1 148 1.0 1 Adherence lipoprotein prediction
ELFCHKLA_00107 VFG042794 BFP 100 7.4e-97 1 181 1.0 1 Adherence minor pilin subunit BfpI prediction
ELFCHKLA_00108 VFG000760 BFP 100 6.6e-101 1 183 1.0 1 Adherence minor pilin subunit BfpJ prediction
ELFCHKLA_00109 VFG042796 BFP 100 1.8e-86 1 162 1.0 1 Adherence minor pilin subunit BfpK prediction
ELFCHKLA_00110 VFG000762 BFP 100 1.1e-70 1 130 1.0 0.8725 Adherence BFP biogenesis protein BfpL prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
ELFCHKLA_00062 CAS07513.1|GH23 100 3.49e-124 1 169 1 1
ELFCHKLA_00106 CAS07448.1|GH23 100 2.28e-103 1 148 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
ELFCHKLA_00099 1.B.22.7.1 100 3e-301 1 552 1.0000 1.0000 1 Channels/Pores 1.B β-Barrel Porins 1.B.22 The Outer Bacterial Membrane Secretin (Secretin) Family