Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C863
  Reference Plasmid   NZ_CP071983.1
  Reference Plasmid Size   9259
  Reference Plasmid GC Content   0.34
  Reference Plasmid Mobility Type   mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0185242 BOLHMFFF_00001 388 6 Gut 0.60 protein_coding missense_variant MODERATE 31A>G Ile11Val
M0185243 BOLHMFFF_00001 390 6 Gut 0.60 protein_coding synonymous_variant LOW 33C>T Ile11Ile
M0185244 BOLHMFFF_00001 492 5 Gut 0.50 protein_coding synonymous_variant LOW 135A>G Leu45Leu
M0185245 BOLHMFFF_00001 514 6 Gut 0.60 protein_coding missense_variant MODERATE 157G>A Val53Met
M0185246 BOLHMFFF_00001 516 6 Gut 0.60 protein_coding synonymous_variant LOW 159G>A Val53Val
M0185247 BOLHMFFF_00001 552 6 Gut 0.60 protein_coding synonymous_variant LOW 195G>A Glu65Glu
M0185248 BOLHMFFF_00001 553 6 Gut 0.60 protein_coding synonymous_variant LOW 196T>C Leu66Leu
M0185249 BOLHMFFF_00001 564 6 Gut 0.60 protein_coding synonymous_variant LOW 207G>A Glu69Glu
M0185250 BOLHMFFF_00001 567 6 Gut 0.60 protein_coding synonymous_variant LOW 210G>A Leu70Leu
M0185251 BOLHMFFF_00002 973 6 Gut 0.60 protein_coding synonymous_variant LOW 279T>C Asn93Asn
M0185252 BOLHMFFF_00002 976 6 Gut 0.60 protein_coding synonymous_variant LOW 282T>C Ile94Ile
M0185253 BOLHMFFF_00002 979 6 Gut 0.60 protein_coding synonymous_variant LOW 285G>A Leu95Leu
M0185254 BOLHMFFF_00002 1030 6 Gut 0.60 protein_coding synonymous_variant LOW 336C>T Pro112Pro
M0185255 BOLHMFFF_00002 1156 6 Gut 0.60 protein_coding synonymous_variant LOW 462G>A Glu154Glu
M0185256 BOLHMFFF_00002 1361 6 Gut 0.60 protein_coding missense_variant MODERATE 667A>G Lys223Glu
M0185257 BOLHMFFF_00002 1457 6 Gut 0.60 protein_coding missense_variant MODERATE 763A>G Lys255Glu
M0185258 BOLHMFFF_00002 1461 6 Gut 0.60 protein_coding missense_variant MODERATE 767G>T Ser256Ile
M0185259 BOLHMFFF_00002 1481 6 Gut 0.60 protein_coding missense_variant MODERATE 787C>T Pro263Ser
M0185260 BOLHMFFF_00002 1496 6 Gut 0.60 protein_coding missense_variant MODERATE 802A>G Asn268Asp
M0185261 BOLHMFFF_00002 1771 6 Gut 0.60 protein_coding synonymous_variant LOW 1077T>C Ser359Ser
M0185262 BOLHMFFF_00002 1837 6 Gut 0.60 protein_coding synonymous_variant LOW 1143G>A Thr381Thr
M0185263 BOLHMFFF_00002 1871 6 Gut 0.60 protein_coding missense_variant MODERATE 1177T>A Ser393Thr
M0185264 BOLHMFFF_00002 1913 6 Gut 0.60 protein_coding missense_variant MODERATE 1219A>G Thr407Ala
M0185265 BOLHMFFF_00002 2147 5 Gut 0.50 protein_coding missense_variant MODERATE 1453C>T Leu485Phe
M0185266 BOLHMFFF_00001 155 5 Gut 0.50 protein_coding upstream_gene_variant MODIFIER -203T>C None
M0185267 BOLHMFFF_00001 227 5 Gut 0.50 protein_coding upstream_gene_variant MODIFIER -131A>G None
M0185268 BOLHMFFF_00001 242 5 Gut 0.50 protein_coding upstream_gene_variant MODIFIER -116C>T None
M0185269 BOLHMFFF_00001 244 5 Gut 0.50 protein_coding upstream_gene_variant MODIFIER -114T>C None
M0185270 BOLHMFFF_00001 270 4 Gut 0.40 protein_coding upstream_gene_variant MODIFIER -88A>G None
M0185271 BOLHMFFF_00001 437 4 Gut 0.40 protein_coding missense_variant MODERATE 80A>C Asn27Thr
M0185272 BOLHMFFF_00001 438 4 Gut 0.40 protein_coding synonymous_variant LOW 81T>C Asn27Asn
M0185273 BOLHMFFF_00001 441 4 Gut 0.40 protein_coding synonymous_variant LOW 84G>A Gln28Gln
M0185274 BOLHMFFF_00002 713 5 Gut 0.50 protein_coding missense_variant MODERATE 19A>G Ser7Gly
M0185275 BOLHMFFF_00002 856 5 Gut 0.50 protein_coding synonymous_variant LOW 162G>A Lys54Lys
M0185276 BOLHMFFF_00002 865 5 Gut 0.50 protein_coding synonymous_variant LOW 171A>G Val57Val
M0185277 BOLHMFFF_00002 937 5 Gut 0.50 protein_coding missense_variant MODERATE 243C>A Asn81Lys
M0185278 BOLHMFFF_00002 944 5 Gut 0.50 protein_coding synonymous_variant LOW 250T>C Leu84Leu
M0185279 BOLHMFFF_00002 1120 5 Gut 0.50 protein_coding synonymous_variant LOW 426T>C Asn142Asn
M0185280 BOLHMFFF_00002 1121 5 Gut 0.50 protein_coding synonymous_variant LOW 427C>T Leu143Leu
M0185281 BOLHMFFF_00002 1261 5 Gut 0.50 protein_coding synonymous_variant LOW 567G>A Lys189Lys
M0185282 BOLHMFFF_00002 1315 5 Gut 0.50 protein_coding synonymous_variant LOW 621A>G Glu207Glu
M0185283 BOLHMFFF_00002 1351 5 Gut 0.50 protein_coding synonymous_variant LOW 657G>A Lys219Lys
M0185284 BOLHMFFF_00002 1547 5 Gut 0.50 protein_coding missense_variant MODERATE 853G>A Val285Ile
M0185285 BOLHMFFF_00002 1586 5 Gut 0.50 protein_coding missense_variant MODERATE 892A>G Thr298Ala
M0185286 BOLHMFFF_00002 1597 5 Gut 0.50 protein_coding missense_variant MODERATE 903A>C Arg301Ser
M0185287 BOLHMFFF_00002 1609 5 Gut 0.50 protein_coding synonymous_variant LOW 915C>T Asp305Asp
M0185288 BOLHMFFF_00002 1610 5 Gut 0.50 protein_coding missense_variant MODERATE 916C>T Leu306Phe
M0185289 BOLHMFFF_00002 1623 4 Gut 0.40 protein_coding missense_variant MODERATE 929C>T Pro310Leu
M0185290 BOLHMFFF_00002 1640 4 Gut 0.40 protein_coding missense_variant MODERATE 946A>G Ser316Gly
M0185291 BOLHMFFF_00002 1682 4 Gut 0.40 protein_coding missense_variant MODERATE 988A>G Arg330Gly
M0185292 BOLHMFFF_00002 1685 4 Gut 0.40 protein_coding missense_variant MODERATE 991T>C Ser331Pro
M0185293 BOLHMFFF_00002 1713 5 Gut 0.50 protein_coding missense_variant MODERATE 1019A>G Asn340Ser
M0185294 BOLHMFFF_00002 1912 4 Gut 0.40 protein_coding synonymous_variant LOW 1218T>C Asn406Asn
M0185295 BOLHMFFF_00002 2052 4 Gut 0.40 protein_coding missense_variant MODERATE 1358G>C Arg453Thr
M0185296 BOLHMFFF_00002 2063 4 Gut 0.40 protein_coding missense_variant MODERATE 1369A>G Lys457Glu
M0185297 BOLHMFFF_00002 2070 4 Gut 0.40 protein_coding missense_variant MODERATE 1376C>T Ala459Val
M0185298 BOLHMFFF_00002 2075 4 Gut 0.40 protein_coding missense_variant MODERATE 1381G>A Glu461Lys
M0185299 BOLHMFFF_00002 2077 4 Gut 0.40 protein_coding missense_variant MODERATE 1383G>C Glu461Asp
M0185300 BOLHMFFF_00007 8929 3 Gut 0.30 protein_coding synonymous_variant LOW 114G>A Gln38Gln
M0185301 BOLHMFFF_00005 9047 3 Gut 0.30 protein_coding upstream_gene_variant MODIFIER -3074T>C None
M0185302 BOLHMFFF_00008 9149 4 Gut 0.40 protein_coding synonymous_variant LOW 79T>C Leu27Leu
M0185303 BOLHMFFF_00008 9150 4 Gut 0.40 protein_coding synonymous_variant LOW 78G>A Glu26Glu
M0185304 BOLHMFFF_00008 9172 4 Gut 0.40 protein_coding missense_variant MODERATE 56G>A Ser19Asn
M0185305 BOLHMFFF_00008 9183 4 Gut 0.40 protein_coding synonymous_variant LOW 45A>G Glu15Glu
M0185306 BOLHMFFF_00003 3722 3 Gut 0.30 protein_coding synonymous_variant LOW 813T>C Gly271Gly
M0185307 BOLHMFFF_00004 3968 4 Gut 0.40 protein_coding upstream_gene_variant MODIFIER -6T>C None
M0185308 BOLHMFFF_00004 4564 4 Gut 0.40 protein_coding synonymous_variant LOW 591A>G Leu197Leu
M0185309 BOLHMFFF_00004 4822 3 Gut 0.30 protein_coding synonymous_variant LOW 849C>T Ile283Ile
M0185310 BOLHMFFF_00004 4839 4 Gut 0.40 protein_coding missense_variant MODERATE 866T>C Val289Ala
M0185311 BOLHMFFF_00004 4891 3 Gut 0.30 protein_coding synonymous_variant LOW 918A>T Ile306Ile
M0185312 BOLHMFFF_00004 4915 3 Gut 0.30 protein_coding synonymous_variant LOW 942C>T Tyr314Tyr
M0185313 BOLHMFFF_00004 4918 4 Gut 0.40 protein_coding missense_variant MODERATE 945G>A Met315Ile






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term