Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C922
  Reference Plasmid   NZ_CP078510.1
  Reference Plasmid Size   261166
  Reference Plasmid GC Content   0.57
  Reference Plasmid Mobility Type   mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0104812 MHPEOBPL_00095 63158 21 Skin 0.36 protein_coding synonymous_variant LOW 183T>G Leu61Leu
M0104813 MHPEOBPL_00095 63176 21 Skin 0.36 protein_coding synonymous_variant LOW 201A>G Gln67Gln
M0104814 MHPEOBPL_00095 63231 21 Skin 0.36 protein_coding missense_variant MODERATE 256A>G Thr86Ala
M0104815 MHPEOBPL_00096 63387 21 Skin 0.36 protein_coding missense_variant MODERATE 38C>T Ala13Val
M0104816 MHPEOBPL_00097 64342 19 Skin 0.32 protein_coding missense_variant MODERATE 613A>G Ile205Val
M0104817 MHPEOBPL_00097 64414 19 Skin 0.32 protein_coding missense_variant MODERATE 685G>A Val229Ile
M0104818 MHPEOBPL_00097 64680 19 Skin 0.32 protein_coding synonymous_variant LOW 951G>A Ala317Ala
M0104819 MHPEOBPL_00097 64879 17 Skin 0.29 protein_coding missense_variant MODERATE 1150G>A Ala384Thr
M0104820 MHPEOBPL_00097 65168 17 Skin 0.29 protein_coding missense_variant MODERATE 1439T>C Leu480Pro
M0104821 MHPEOBPL_00126 96209 8 Skin 0.14 protein_coding downstream_gene_variant MODIFIER *4903A>G None
M0104822 MHPEOBPL_00126 96283 8 Skin 0.14 protein_coding downstream_gene_variant MODIFIER *4977G>T None
M0104823 MHPEOBPL_00127 96391 7 Skin 0.12 protein_coding downstream_gene_variant MODIFIER *4318A>G None
M0104824 MHPEOBPL_00133 99261 3 Skin 0.05 protein_coding synonymous_variant LOW 267T>C Asn89Asn
M0104825 MHPEOBPL_00134 99633 3 Skin 0.05 protein_coding missense_variant MODERATE 1396T>A Ser466Thr
M0104826 MHPEOBPL_00134 99634 3 Skin 0.05 protein_coding synonymous_variant LOW 1395A>G Ala465Ala
M0104827 MHPEOBPL_00134 99637 3 Skin 0.05 protein_coding synonymous_variant LOW 1392A>C Thr464Thr
M0104828 MHPEOBPL_00134 99639 3 Skin 0.05 protein_coding missense_variant MODERATE 1390A>G Thr464Ala
M0104829 MHPEOBPL_00134 99643 3 Skin 0.05 protein_coding synonymous_variant LOW 1386G>C Arg462Arg
M0104830 MHPEOBPL_00134 99646 3 Skin 0.05 protein_coding synonymous_variant LOW 1383A>G Ala461Ala
M0104831 MHPEOBPL_00134 99820 3 Skin 0.05 protein_coding synonymous_variant LOW 1209A>G Pro403Pro
M0104832 MHPEOBPL_00134 99841 3 Skin 0.05 protein_coding synonymous_variant LOW 1188A>C Gly396Gly
M0104833 MHPEOBPL_00134 100016 3 Skin 0.05 protein_coding missense_variant MODERATE 1013A>G Tyr338Cys
M0104834 MHPEOBPL_00134 100105 3 Skin 0.05 protein_coding synonymous_variant LOW 924T>A Pro308Pro






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group
MHPEOBPL_00001 Mercury (Hg) 76.7 6.6e-50 1 116 1.0000 1.0000 experiment
MHPEOBPL_00003 Mercury (Hg), Phenylmercury Acetate [class: Organo-mercury], Methylmercury Acetate [class: Organo-mercury] 74.5 1.5e-235 1 562 1.0125 1.0000 experiment
MHPEOBPL_00024 Mercury (Hg), Phenylmercury Acetate [class: Organo-mercury], Methylmercury Acetate [class: Organo-mercury] 98.6 2.9e-120 1 212 1.0000 1.0000 experiment
MHPEOBPL_00025 Mercury (Hg), Phenylmercury Acetate [class: Organo-mercury] 99.1 1.2e-118 1 217 1.0000 1.0000 experiment
MHPEOBPL_00026 Mercury (Hg), Phenylmercury Acetate [class: Organo-mercury], Methylmercury Acetate [class: Organo-mercury] 98.6 3.50000000012062e-314 1 569 1.0000 1.0000 experiment
MHPEOBPL_00027 Mercury (Hg) 100 1.2e-43 1 91 1.0000 1.0000 experiment
MHPEOBPL_00028 Mercury (Hg) 100 1.2e-62 1 116 1.0000 1.0000 experiment
MHPEOBPL_00029 Mercury (Hg) 93.8 4e-73 1 144 1.0000 1.0000 experiment
MHPEOBPL_00291 Chromium (Cr) 76.1 3.3e-81 1 188 0.9447 0.9543 experiment
MHPEOBPL_00292 Chromium (Cr) 99.8 1.5e-229 1 416 1.0000 1.0000 experiment
MHPEOBPL_00300 Benzylkonium Chloride (BAC) [class: Quaternary Ammonium Compounds (QACs)], Ethidium Bromide [class: Phenanthridine], Acriflavine [class: Acridine], Chlorhexidine [class: Biguanides], Pyronin Y [class: Xanthene], Rhodamine 6G [class: Xanthene], Methyl Viologen [class: Paraquat], Tetraphenylphosphonium (TPP) [class: Quaternary Ammonium Compounds (QACs)], 4,6-diamidino-2-phenylindole (DAPI) [class: Diamindine], Acridine Orange [class: Acridine], Sodium Dodecyl Sulfate (SDS) [class: Organo-sulfate], Sodium Deoxycholate (SDC) [class: Acid], Crystal Violet [class: Triarylmethane], Cetrimide (CTM) [class: Quaternary Ammonium Compounds (QACs)], Cetylpyridinium Chloride (CPC) [class: Quaternary Ammonium Compounds (QACs)], Dequalinium [class: Quaternary Ammonium Compounds (QACs)] 100 1.1e-60 1 115 1.0000 1.0000 experiment
MHPEOBPL_00309 Mercury (Hg), Phenylmercury Acetate [class: Organo-mercury] 72.2 3.2e-54 1 133 0.9366 0.9236 experiment
MHPEOBPL_00001 Mercury (Hg) 78.4 6.8e-48 1 116 1.0000 1.0000 prediction
MHPEOBPL_00003 Mercury (Hg), Phenylmercury Acetate [class: Organo-mercury] 75.2 7.2e-236 1 562 1.0125 1.0000 prediction
MHPEOBPL_00016 Copper (Cu) 91.7 2.7e-233 1 457 1.0000 1.0000 prediction
MHPEOBPL_00024 Mercury (Hg), Phenylmercury Acetate [class: Organo-mercury] 99.1 2.3e-118 1 212 1.0000 1.0000 prediction
MHPEOBPL_00025 Mercury (Hg), Phenylmercury Acetate [class: Organo-mercury] 99.1 2.9e-116 1 217 1.0000 1.0000 prediction
MHPEOBPL_00026 Mercury (Hg), Phenylmercury Acetate [class: Organo-mercury] 99.6 0 1 569 1.0000 1.0000 prediction
MHPEOBPL_00027 Mercury (Hg) 100 2.8e-41 1 91 1.0000 0.8835 prediction
MHPEOBPL_00028 Mercury (Hg) 99.1 3.5e-60 1 116 1.0000 1.0000 prediction
MHPEOBPL_00029 Mercury (Hg) 100 1.9e-76 1 144 1.0000 1.0000 prediction
MHPEOBPL_00290 Chromium (Cr) 72.1 6.2e-54 1 136 0.9128 0.9067 prediction
MHPEOBPL_00291 Chromium (Cr) 100 5.5e-114 1 199 1.0000 1.0000 prediction
MHPEOBPL_00292 Chromium (Cr) 99.8 3.4e-227 1 416 1.0000 1.0000 prediction
MHPEOBPL_00293 Chromium (Cr), Methyl Viologen [class: Paraquat], Menadione [class: Naphthoquinone] 100 2.9e-178 1 314 1.0000 1.0000 prediction






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
MHPEOBPL_00299 ARO:3000410 100 2.94e-199 1 279 1.0000 1.0000 sulfonamide antibiotic sulfonamide resistant sul antibiotic target replacement
MHPEOBPL_00300 ARO:3005010 100 2.01e-78 1 115 1.0000 1.0000 disinfecting agents and antiseptics major facilitator superfamily (MFS) antibiotic efflux pump antibiotic efflux
MHPEOBPL_00301 ARO:3002601 99.6 1.33e-186 1 259 1.0000 0.9848 aminoglycoside antibiotic ANT(3'') antibiotic inactivation






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
MHPEOBPL_00302 PHI:9804 int 100 4e-14 1 37 0.1098 1.0000 rodents gastroenteritis integrase reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
MHPEOBPL_00014 QXP27892.1|GH15 100 0 1 574 1 0.9535
MHPEOBPL_00191 QXP27787.1|GH15 100 0 1 601 1 1
MHPEOBPL_00258 QGZ33004.1|GH23 100 1.61e-132 1 189 1 1
MHPEOBPL_00285 QXP27858.1|GH15 100 0 1 658 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
MHPEOBPL_00001 1.A.72.3.1 73.3 3e-46 1 116 1.0000 1.2747 1 Channels/Pores 1.A α-Type Channels 1.A.72 The Mercuric Ion Pore (Mer) Superfamily
MHPEOBPL_00027 1.A.72.3.1 76.9 2.5e-32 1 91 1.0000 1.0000 1 Channels/Pores 1.A α-Type Channels 1.A.72 The Mercuric Ion Pore (Mer) Superfamily
MHPEOBPL_00028 1.A.72.3.1 87.9 3e-54 1 116 1.0000 1.2747 1 Channels/Pores 1.A α-Type Channels 1.A.72 The Mercuric Ion Pore (Mer) Superfamily
MHPEOBPL_00292 2.A.51.1.3 99.8 5.6e-228 1 416 1.0000 1.0000 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.51 The Chromate Ion Transporter (CHR) Family