Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C981
  Reference Plasmid   NZ_CP089851.1
  Reference Plasmid Size   359787
  Reference Plasmid GC Content   0.32
  Reference Plasmid Mobility Type   mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0191617 HIPHBLPG_00170 179555 3 Gut 0.25 protein_coding upstream_gene_variant MODIFIER -4609T>C None
M0191618 HIPHBLPG_00176 179973 3 Gut 0.25 protein_coding synonymous_variant LOW 684T>C Asn228Asn
M0191619 HIPHBLPG_00176 179974 3 Gut 0.25 protein_coding missense_variant MODERATE 683A>G Asn228Ser
M0191620 HIPHBLPG_00176 179978 3 Gut 0.25 protein_coding missense_variant MODERATE 679G>A Val227Ile
M0191621 HIPHBLPG_00177 180743 3 Gut 0.25 protein_coding missense_variant MODERATE 1220C>A Pro407His
M0191622 HIPHBLPG_00177 181619 3 Gut 0.25 protein_coding missense_variant MODERATE 344T>A Ile115Asn
M0191623 HIPHBLPG_00185 184808 3 Gut 0.25 protein_coding synonymous_variant LOW 225G>A Val75Val
M0191624 HIPHBLPG_00177 185701 4 Gut 0.33 protein_coding upstream_gene_variant MODIFIER -3739A>G None
M0191625 HIPHBLPG_00186 185915 3 Gut 0.25 protein_coding missense_variant MODERATE 457G>A Glu153Lys
M0191626 HIPHBLPG_00173 177950 3 Gut 0.25 protein_coding synonymous_variant LOW 984A>G Lys328Lys






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group
HIPHBLPG_00006 Arsenic (As), Antimony (Sb) 78.6 2.5e-132 16 328 0.9485 0.8792 prediction






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
HIPHBLPG_00012 ARO:3002626 100 9.66e-218 1 287 1.0000 1.0000 aminoglycoside antibiotic ANT(6) antibiotic inactivation
HIPHBLPG_00015 ARO:3002597 100 0 1 479 1.0000 1.0000 aminoglycoside antibiotic aminoglycoside bifunctional resistance protein antibiotic inactivation






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
HIPHBLPG_00320 PHI:124066 prdB 74 1.5e-61 1 150 1.0000 0.9585 rodents nosocomial diarrhea proline reductase reduced virulence
HIPHBLPG_00321 PHI:124066 prdB 79 4.3e-31 1 81 1.0000 0.9585 rodents nosocomial diarrhea proline reductase reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
HIPHBLPG_00236 UHL93707.1|GH25 100 7.27e-241 1 309 1 1
HIPHBLPG_00246 UHL93717.1|GH25 100 0 1 783 1 1
HIPHBLPG_00248 UHL93719.1|GH23 100 0 1 1442 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
HIPHBLPG_00006 2.A.59.1.6 76.4 3.9e-131 16 328 0.9485 1.0574 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.59 The Arsenical Resistance-3 (ACR3) Family
HIPHBLPG_00197 2.A.21.3.20 74.7 9.2e-209 1 498 0.9940 0.9960 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.21 The Solute:Sodium Symporter (SSS) Family
HIPHBLPG_00343 2.A.1.1.74 71.2 2.2e-171 2 417 0.9928 0.9882 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.1 The Major Facilitator Superfamily (MFS)