Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C998
  Reference Plasmid   NZ_CP091207.1
  Reference Plasmid Size   138406
  Reference Plasmid GC Content   0.35
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0191756 EHKNFLEE_00019 16790 4 Gut 0.40 protein_coding missense_variant MODERATE 2048C>T Thr683Ile
M0191757 EHKNFLEE_00020 17525 4 Gut 0.40 protein_coding synonymous_variant LOW 177T>G Gly59Gly
M0191758 EHKNFLEE_00020 17597 4 Gut 0.40 protein_coding synonymous_variant LOW 249T>C Ala83Ala
M0191759 EHKNFLEE_00018 14314 3 Gut 0.30 protein_coding synonymous_variant LOW 1635T>A Ala545Ala
M0191760 EHKNFLEE_00018 14371 3 Gut 0.30 protein_coding synonymous_variant LOW 1692C>G Thr564Thr
M0191761 EHKNFLEE_00018 14375 3 Gut 0.30 protein_coding missense_variant MODERATE 1696A>G Met566Val
M0191762 EHKNFLEE_00024 21539 3 Gut 0.30 protein_coding synonymous_variant LOW 654T>G Gly218Gly
M0191763 EHKNFLEE_00063 53846 3 Gut 0.30 protein_coding synonymous_variant LOW 168T>C Val56Val






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
EHKNFLEE_00009 VFG042989 PilA-type pili (PGS1, pilin gene clusters 1) 99.1 4.7e-125 1 223 1.0 1 Adherence putative housekeeping sortase prediction
EHKNFLEE_00010 VFG042988 PilA-type pili (PGS1, pilin gene clusters 1) 99.1 0 1 658 1.0 1 Adherence PilA prediction
EHKNFLEE_00011 VFG042987 PilA-type pili (PGS1, pilin gene clusters 1) 100 1.3e-139 1 250 1.0 1 Adherence putative pilus-dedicated sortase prediction
EHKNFLEE_00012 VFG042986 PilA-type pili (PGS1, pilin gene clusters 1) 97.6 9.3e-130 1 253 1.0 1.008 Adherence cell wall-associated LPXTG-like protein prediction
EHKNFLEE_00013 VFG042985 PilA-type pili (PGS1, pilin gene clusters 1) 98.8 4.9e-38 1 86 1.0 1 Adherence hypothetical hydrophobic peptide prediction
EHKNFLEE_00014 VFG042984 PilA-type pili (PGS1, pilin gene clusters 1) 99.3 0 1 696 1.0 1 Adherence minor pilin subunit prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
EHKNFLEE_00067 PHI:5205 HMPREF0351_10118 (WxL locusC) 71.8 1.2e-168 1 389 1.0000 0.9949 rodents endocarditis involved in bile salt stress and endocarditis pathogenesis reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
EHKNFLEE_00025 AOT80009.1|GH73 96.3 2.78e-271 1 401 1 1
EHKNFLEE_00076 AOM14759.1|GH13_18 99.8 0 1 486 1 1
EHKNFLEE_00077 QDA39721.1|GH13_31 99.4 2.25e-237 1 322 1 1
EHKNFLEE_00079 QOG29405.1|GH36 100 0 1 727 1 1
EHKNFLEE_00083 AWV59846.1|GH4 100 0 1 436 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
EHKNFLEE_00080 3.A.1.1.28 75.3 5.4e-118 5 276 0.9855 0.7294 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily
EHKNFLEE_00081 3.A.1.1.28 73.8 8.2e-125 1 290 1.0000 0.7692 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily