Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C999
  Reference Plasmid   NZ_CP091649.1
  Reference Plasmid Size   448767
  Reference Plasmid GC Content   0.62
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0106221 MPLOPBMF_00467 434901 4 Skin 0.50 protein_coding upstream_gene_variant MODIFIER -4883A>C None
M0106222 MPLOPBMF_00475 434993 4 Skin 0.50 protein_coding missense_variant MODERATE 83G>T Cys28Phe
M0106223 MPLOPBMF_00475 435062 4 Skin 0.50 protein_coding missense_variant MODERATE 152G>T Arg51Leu
M0106224 MPLOPBMF_00475 435269 4 Skin 0.50 protein_coding missense_variant MODERATE 359T>C Met120Thr
M0106225 MPLOPBMF_00475 435272 4 Skin 0.50 protein_coding missense_variant MODERATE 362C>T Ala121Val
M0106226 MPLOPBMF_00475 435286 4 Skin 0.50 protein_coding missense_variant MODERATE 376A>G Lys126Glu
M0106227 MPLOPBMF_00475 435376 4 Skin 0.50 protein_coding missense_variant MODERATE 466G>A Gly156Ser
M0106228 MPLOPBMF_00475 435529 4 Skin 0.50 protein_coding missense_variant MODERATE 619G>A Asp207Asn
M0106229 MPLOPBMF_00475 435631 4 Skin 0.50 protein_coding missense_variant MODERATE 721T>G Ser241Ala
M0106230 MPLOPBMF_00475 435648 4 Skin 0.50 protein_coding missense_variant MODERATE 738T>A Phe246Leu
M0106231 MPLOPBMF_00475 435793 4 Skin 0.50 protein_coding missense_variant MODERATE 883C>G Leu295Val
M0106232 MPLOPBMF_00469 435836 4 Skin 0.50 protein_coding upstream_gene_variant MODIFIER -4932C>A None
M0106233 MPLOPBMF_00476 436052 4 Skin 0.50 protein_coding missense_variant MODERATE 122G>C Cys41Ser
M0106234 MPLOPBMF_00476 436212 4 Skin 0.50 protein_coding missense_variant MODERATE 282G>T Met94Ile
M0106235 MPLOPBMF_00476 436449 4 Skin 0.50 protein_coding synonymous_variant LOW 519A>G Pro173Pro
M0106236 MPLOPBMF_00476 436675 4 Skin 0.50 protein_coding missense_variant MODERATE 745A>G Ile249Val
M0106237 MPLOPBMF_00471 436804 4 Skin 0.50 protein_coding upstream_gene_variant MODIFIER -4741G>T None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group
MPLOPBMF_00165 Arsenic (As) 70.9 2.2e-92 5 240 0.9875 1.0216 experiment
MPLOPBMF_00178 Cadmium (Cd), Zinc (Zn), Cobalt (Co) 73.9 0 1 1043 0.9748 0.9812 experiment
MPLOPBMF_00179 Cadmium (Cd), Zinc (Zn) 86.3 8.8e-115 1 226 0.9956 0.9869 experiment
MPLOPBMF_00220 Mercury (Hg) 79.5 1.5e-248 1 559 1.0036 1.0000 experiment
MPLOPBMF_00223 Mercury (Hg) 79.1 4.5e-49 7 121 0.9504 0.9914 experiment
MPLOPBMF_00224 Mercury (Hg), Phenylmercury Acetate [class: Organo-mercury] 79.7 3.2e-59 1 133 0.9568 0.9236 experiment
MPLOPBMF_00157 Copper (Cu) 72.6 2.1e-195 3 430 1.0000 0.9131 prediction
MPLOPBMF_00158 Cadmium (Cd), Zinc (Zn), Cobalt (Co) 83.1 9.2e-227 1 498 1.0000 1.0440 prediction
MPLOPBMF_00162 Cadmium (Cd), Zinc (Zn) 80.3 3.4e-207 1 461 0.9935 0.9893 prediction
MPLOPBMF_00165 Arsenic (As) 91.2 1.9e-124 1 240 1.0000 1.0000 prediction
MPLOPBMF_00166 Arsenic (As) 74.3 2.8e-56 1 140 1.0000 0.9929 prediction
MPLOPBMF_00178 Cadmium (Cd), Zinc (Zn), Cobalt (Co) 74.6 0 1 1043 0.9748 0.9793 prediction
MPLOPBMF_00179 Cadmium (Cd), Zinc (Zn) 99.6 1.1e-126 1 227 1.0000 0.9956 prediction
MPLOPBMF_00180 Cadmium (Cd), Zinc (Zn) 100 1.1e-258 1 468 1.0000 1.0000 prediction
MPLOPBMF_00219 Mercury (Hg), Phenylmercury Acetate [class: Organo-mercury] 100 1.4e-120 1 213 1.0000 1.0000 prediction
MPLOPBMF_00220 Mercury (Hg), Phenylmercury Acetate [class: Organo-mercury] 100 1.79999999998439e-313 1 559 1.0000 1.0000 prediction
MPLOPBMF_00222 Mercury (Hg) 100 9.7e-42 1 91 1.0000 1.0000 prediction
MPLOPBMF_00223 Mercury (Hg) 100 1.1e-61 1 121 1.0000 1.0000 prediction
MPLOPBMF_00224 Mercury (Hg) 100 8.6e-74 1 139 1.0000 1.0000 prediction
MPLOPBMF_00226 Mercury (Hg) 71.7 4.2e-52 1 136 0.9857 0.9787 prediction






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
MPLOPBMF_00065 QUN49916.1|GH23 92.9 4.59e-116 1 184 1 1
MPLOPBMF_00082 UKD18098.1|GH23 100 4.82e-159 1 235 1 1
MPLOPBMF_00387 UKD17955.1|GH23 100 8.12e-193 1 269 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
MPLOPBMF_00118 2.A.55.3.6 72.2 7.1e-210 16 553 0.9729 0.9761 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.55 The Metal Ion (Mn2+-iron) Transporter (Nramp) Family
MPLOPBMF_00178 2.A.6.1.2 73.9 0 1 1043 0.9748 0.9755 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.6 The Resistance-Nodulation-Cell Division (RND) Superfamily
MPLOPBMF_00182 1.B.1.4.6 95.2 5.8e-134 139 386 0.6425 1.0000 1 Channels/Pores 1.B β-Barrel Porins 1.B.1 The General Bacterial Porin (GBP) Family
MPLOPBMF_00223 1.A.72.3.1 79.1 8.3e-47 7 121 0.9504 1.2637 1 Channels/Pores 1.A α-Type Channels 1.A.72 The Mercuric Ion Pore (Mer) Superfamily
MPLOPBMF_00475 3.A.1.9.4 98.3 1.6e-168 1 298 1.0000 1.1418 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily
MPLOPBMF_00476 3.A.1.9.4 99.3 5.3e-153 1 282 1.0000 1.0805 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily
MPLOPBMF_00477 3.A.1.9.4 97.4 1.6e-119 1 227 0.8534 0.8697 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily