Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C1019
  Reference Plasmid   NZ_CP095602.1
  Reference Plasmid Size   152803
  Reference Plasmid GC Content   0.52
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0191861 BOOAKKFM_00127 105554 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -384C>T None
M0191862 BOOAKKFM_00129 105902 3 Gut 0.75 protein_coding missense_variant MODERATE 148C>A Leu50Ile
M0191863 BOOAKKFM_00129 105994 3 Gut 0.75 protein_coding missense_variant MODERATE 56A>C Lys19Thr
M0191864 BOOAKKFM_00129 106033 3 Gut 0.75 protein_coding missense_variant MODERATE 17G>A Arg6Gln
M0191865 BOOAKKFM_00129 106035 3 Gut 0.75 protein_coding synonymous_variant LOW 15C>A Ser5Ser
M0191866 BOOAKKFM_00127 106060 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -890A>T None
M0191867 BOOAKKFM_00127 106100 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -930T>A None
M0191868 BOOAKKFM_00127 106103 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -933A>G None
M0191869 BOOAKKFM_00127 106105 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -935T>C None
M0191870 BOOAKKFM_00127 106110 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -940G>A None
M0191871 BOOAKKFM_00127 106118 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -948T>C None
M0191872 BOOAKKFM_00127 106119 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -949G>A None
M0191873 BOOAKKFM_00127 106125 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -955G>A None
M0191874 BOOAKKFM_00127 106178 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -1008C>T None
M0191875 BOOAKKFM_00127 106180 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -1010C>A None
M0191876 BOOAKKFM_00127 106181 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -1011T>C None
M0191877 BOOAKKFM_00127 106182 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -1012T>A None
M0191878 BOOAKKFM_00127 106197 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -1027T>A None
M0191879 BOOAKKFM_00127 106233 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -1063T>C None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
BOOAKKFM_00019 ARO:3004621 99 6.49e-211 1 286 1.0000 1.0000 aminoglycoside antibiotic AAC(3) antibiotic inactivation
BOOAKKFM_00022 ARO:3001396 100 6.06e-199 16 291 0.9485 1.0000 cephalosporin OXA beta-lactamase antibiotic inactivation
BOOAKKFM_00023 ARO:3002581 98 1.4e-140 2 199 0.9950 0.9754 aminoglycoside antibiotic AAC(6') antibiotic inactivation
BOOAKKFM_00030 ARO:3001878 100 1.53e-195 1 280 1.0000 0.9622 cephalosporin CTX-M beta-lactamase antibiotic inactivation
BOOAKKFM_00039 ARO:3002798 91.3 3.33e-100 1 173 0.9301 0.7936 fluoroquinolone antibiotic quinolone resistance protein (qnr) antibiotic target protection
BOOAKKFM_00048 ARO:3002714 99.4 3.38e-65 1 156 1.0000 0.7290 fluoroquinolone antibiotic quinolone resistance protein (qnr) antibiotic target protection
BOOAKKFM_00055 ARO:3003741 100 1.87e-214 1 294 1.0000 1.0000 macrolide antibiotic macrolide phosphotransferase (MPH) antibiotic inactivation
BOOAKKFM_00056 ARO:3003109 100 0 1 491 1.0000 1.0000 macrolide antibiotic msr-type ABC-F protein antibiotic target protection
BOOAKKFM_00059 ARO:3000858 100 4.87e-187 1 257 1.0000 1.0000 aminoglycoside antibiotic 16S rRNA methyltransferase (G1405) antibiotic target alteration
BOOAKKFM_00063 ARO:3000410 100 2.94e-199 1 279 1.0000 1.0000 sulfonamide antibiotic sulfonamide resistant sul antibiotic target replacement
BOOAKKFM_00067 ARO:3001205 100 1.67e-91 1 121 1.0000 1.0000 glycopeptide antibiotic Bleomycin resistant protein antibiotic inactivation
BOOAKKFM_00068 ARO:3000589 100 4.69e-196 1 270 1.0000 1.0000 carbapenem NDM beta-lactamase antibiotic inactivation






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
BOOAKKFM_00065 PHI:10922 ampR 100 4.9e-170 1 291 1.0000 1.0000 rodents pneumonia regulator of the LysR family reduced virulence
BOOAKKFM_00068 PHI:6347 NDM-1 100 4.1e-155 1 270 1.0000 1.0000 moths infection carbapenemase unaffected pathogenicity






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
BOOAKKFM_00137 QJO48544.1|GH23 99.4 2.6e-112 1 159 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
BOOAKKFM_00128 1.E.53.1.7 72 9e-14 1 50 0.9434 0.7042 1 Channels/Pores 1.E Holins 1.E.53 The Toxic Hok/Gef Protein (Hok/Gef) Family