Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C1021
  Reference Plasmid   NZ_CP096212.1
  Reference Plasmid Size   291523
  Reference Plasmid GC Content   0.36
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0191880 OFCAOKOE_00227 199825 3 Gut 0.18 protein_coding missense_variant MODERATE 514A>T Ile172Leu
M0191881 OFCAOKOE_00227 199832 3 Gut 0.18 protein_coding synonymous_variant LOW 507C>T Phe169Phe
M0191882 OFCAOKOE_00227 199840 3 Gut 0.18 protein_coding missense_variant MODERATE 499A>T Thr167Ser






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
OFCAOKOE_00247 VFG042984 PilA-type pili (PGS1, pilin gene clusters 1) 99 0 1 695 0.9986 0.9986 Adherence minor pilin subunit prediction
OFCAOKOE_00248 VFG042985 PilA-type pili (PGS1, pilin gene clusters 1) 96.5 2.4e-37 1 86 1.0 1 Adherence hypothetical hydrophobic peptide prediction
OFCAOKOE_00249 VFG042986 PilA-type pili (PGS1, pilin gene clusters 1) 98.4 1.1e-130 1 253 1.0 1.008 Adherence cell wall-associated LPXTG-like protein prediction
OFCAOKOE_00250 VFG042987 PilA-type pili (PGS1, pilin gene clusters 1) 99.2 1.9e-138 1 250 1.0 1 Adherence putative pilus-dedicated sortase prediction
OFCAOKOE_00251 VFG042988 PilA-type pili (PGS1, pilin gene clusters 1) 95.1 0 1 658 1.0 1 Adherence PilA prediction
OFCAOKOE_00252 VFG042989 PilA-type pili (PGS1, pilin gene clusters 1) 99.1 8e-125 1 223 1.0 1 Adherence putative housekeeping sortase prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
OFCAOKOE_00269 ARO:3002597 100 7.46e-270 6 371 0.9865 0.7641 aminoglycoside antibiotic aminoglycoside bifunctional resistance protein antibiotic inactivation






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
OFCAOKOE_00025 PHI:5205 HMPREF0351_10118 (WxL locusC) 100 1e-226 1 392 1.0000 1.0000 rodents endocarditis involved in bile salt stress and endocarditis pathogenesis reduced virulence
OFCAOKOE_00039 PHI:5205 HMPREF0351_10118 (WxL locusC) 77.9 2e-23 13 80 0.8095 1.0000 rodents endocarditis involved in bile salt stress and endocarditis pathogenesis reduced virulence
OFCAOKOE_00090 PHI:5205 HMPREF0351_10118 (WxL locusC) 71.8 1.2e-168 1 389 1.0000 1.0000 rodents endocarditis involved in bile salt stress and endocarditis pathogenesis reduced virulence
OFCAOKOE_00186 PHI:5205 HMPREF0351_10118 (WxL locusC) 77.6 6.3e-181 1 392 1.0000 1.0000 rodents endocarditis involved in bile salt stress and endocarditis pathogenesis reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
OFCAOKOE_00046 QMV94891.1|GH32 100 0 1 479 1 1
OFCAOKOE_00073 AOM14759.1|GH13_18 100 0 1 486 1 1
OFCAOKOE_00074 QQJ93880.1|GH13_31 99.8 5.12e-306 1 406 1 1
OFCAOKOE_00075 QTO65065.1|GH36 100 0 1 727 1 1
OFCAOKOE_00079 AWV59846.1|GH4 100 0 1 436 1 1
OFCAOKOE_00125 QTO59404.1|GH1 100 0 17 484 0.9669 1
OFCAOKOE_00139 APV52892.1|GH32 100 0 1 490 1 1
OFCAOKOE_00231 AOT80009.1|GH73 96.8 7.13e-274 1 401 1 1
OFCAOKOE_00321 QOJ75557.1|GH36 100 0 1 749 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
OFCAOKOE_00023 1.C.90.3.3 100 2.3e-38 1 87 1.0000 1.0000 1 Channels/Pores 1.C Pore-Forming Toxins (Proteins and Peptides) 1.C.90 The Carnocyclin A (Carnocyclin) Family
OFCAOKOE_00076 3.A.1.1.28 75.3 5.4e-118 5 276 0.9855 0.7294 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily
OFCAOKOE_00077 3.A.1.1.28 74.1 2.8e-125 1 290 1.0000 0.7692 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily