Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C1027
  Reference Plasmid   NZ_CP097071.1
  Reference Plasmid Size   76993
  Reference Plasmid GC Content   0.35
  Reference Plasmid Mobility Type   mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0192552 HEPOEHNA_00054 48535 3 Gut 0.11 protein_coding synonymous_variant LOW 120A>G Gly40Gly
M0192553 HEPOEHNA_00049 49157 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -2606C>T None
M0192554 HEPOEHNA_00055 49431 3 Gut 0.11 protein_coding missense_variant MODERATE 347G>A Arg116His
M0192555 HEPOEHNA_00055 49633 3 Gut 0.11 protein_coding missense_variant MODERATE 145A>G Asn49Asp
M0192556 HEPOEHNA_00055 49727 3 Gut 0.11 protein_coding missense_variant MODERATE 51G>T Gln17His
M0192557 HEPOEHNA_00056 50228 3 Gut 0.11 protein_coding synonymous_variant LOW 322T>C Leu108Leu
M0192558 HEPOEHNA_00056 50395 3 Gut 0.11 protein_coding missense_variant MODERATE 155T>C Ile52Thr
M0192559 HEPOEHNA_00049 50689 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -4138C>T None
M0192560 HEPOEHNA_00049 50743 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -4192A>G None
M0192561 HEPOEHNA_00049 50771 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -4220A>T None
M0192562 HEPOEHNA_00049 50988 4 Gut 0.15 protein_coding upstream_gene_variant MODIFIER -4437G>A None
M0192563 HEPOEHNA_00057 51085 3 Gut 0.11 protein_coding missense_variant MODERATE 49A>G Ile17Val
M0192564 HEPOEHNA_00057 51238 3 Gut 0.11 protein_coding missense_variant MODERATE 202T>C Ser68Pro
M0192565 HEPOEHNA_00049 51475 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -4924A>T None
M0192566 HEPOEHNA_00058 51556 4 Gut 0.15 protein_coding synonymous_variant LOW 78G>A Gly26Gly
M0192567 HEPOEHNA_00059 52352 3 Gut 0.11 protein_coding synonymous_variant LOW 348A>C Gly116Gly
M0192568 HEPOEHNA_00059 52422 3 Gut 0.11 protein_coding missense_variant MODERATE 418A>G Arg140Gly
M0192569 HEPOEHNA_00059 52763 3 Gut 0.11 protein_coding synonymous_variant LOW 759A>G Gly253Gly
M0192570 HEPOEHNA_00060 52943 3 Gut 0.11 protein_coding missense_variant MODERATE 4A>G Ile2Val
M0192571 HEPOEHNA_00060 53113 3 Gut 0.11 protein_coding missense_variant MODERATE 174G>T Glu58Asp
M0192572 HEPOEHNA_00062 54665 3 Gut 0.11 protein_coding missense_variant MODERATE 401C>T Thr134Met
M0192573 HEPOEHNA_00062 54841 3 Gut 0.11 protein_coding missense_variant MODERATE 577T>C Phe193Leu
M0192574 HEPOEHNA_00063 56083 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -35T>C None
M0192575 HEPOEHNA_00063 56420 3 Gut 0.11 protein_coding synonymous_variant LOW 303T>C Ala101Ala
M0192576 HEPOEHNA_00064 57592 3 Gut 0.11 protein_coding missense_variant MODERATE 46A>G Asn16Asp
M0192577 HEPOEHNA_00064 57593 3 Gut 0.11 protein_coding missense_variant MODERATE 47A>G Asn16Ser
M0192578 HEPOEHNA_00065 57829 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -11G>A None
M0192579 HEPOEHNA_00065 58042 3 Gut 0.11 protein_coding missense_variant MODERATE 203T>C Leu68Pro
M0192580 HEPOEHNA_00049 50764 7 Gut 0.26 protein_coding upstream_gene_variant MODIFIER -4213C>T None
M0192581 HEPOEHNA_00059 52361 8 Gut 0.30 protein_coding synonymous_variant LOW 357C>T Asp119Asp
M0192582 HEPOEHNA_00059 52814 8 Gut 0.30 protein_coding synonymous_variant LOW 810C>T Asp270Asp
M0192583 HEPOEHNA_00070 62081 10 Gut 0.37 protein_coding synonymous_variant LOW 1065T>C Asn355Asn
M0192584 HEPOEHNA_00066 59491 3 Gut 0.11 protein_coding missense_variant MODERATE 316T>C Ser106Pro
M0192585 HEPOEHNA_00066 59348 3 Gut 0.11 protein_coding missense_variant MODERATE 173G>A Arg58Lys
M0192586 HEPOEHNA_00065 58160 3 Gut 0.11 protein_coding synonymous_variant LOW 321T>C Asp107Asp






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
HEPOEHNA_00010 VFG002164 AS 95.1 0 1 1305 1.0 1 Adherence aggregation substance PrgB/Asc10 experiment
HEPOEHNA_00072 VFG002162 BSH 73.8 1.9e-147 1 324 1.0 0.9969 Stress survival bile salt hydrolase experiment
HEPOEHNA_00010 VFG002164 AS 95.1 0 1 1305 1.0 1 Adherence aggregation substance PrgB/Asc10 prediction
HEPOEHNA_00072 VFG019385 BSH 74.4 3.3e-148 1 324 1.0 0.9969 Stress survival bile salt hydrolase prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
HEPOEHNA_00030 PHI:2999 EF0026 98.1 3.3e-91 1 162 1.0000 1.0000 moths nosocomial infection None increased virulence (hypervirulence)






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
HEPOEHNA_00016 QBA99744.1|GH73 100 4.65000000000497e-313 1 423 1 1
HEPOEHNA_00063 QZK92746.1|GH1 100 0 1 468 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
HEPOEHNA_00062 4.A.3.1.1 71.9 1.1e-239 1 568 1.0000 0.9965 4 Group Translocators 4.A Phosphotransfer-driven Group Translocators (PTS) 4.A.3 The PTS Lactose-N,N'-Diacetylchitobiose-β-glucoside (Lac) Family