Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C1101
  Reference Plasmid   NZ_CP115216.1
  Reference Plasmid Size   178059
  Reference Plasmid GC Content   0.57
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0107958 DMKGBHCF_00015 15538 5 Skin 0.10 protein_coding synonymous_variant LOW 804T>G Pro268Pro
M0107959 DMKGBHCF_00015 15827 15 Skin 0.31 protein_coding missense_variant MODERATE 515T>C Val172Ala
M0107960 DMKGBHCF_00016 16774 10 Skin 0.20 protein_coding missense_variant MODERATE 572G>A Arg191Gln
M0107961 DMKGBHCF_00018 18382 10 Skin 0.20 protein_coding synonymous_variant LOW 672G>A Gln224Gln
M0107962 DMKGBHCF_00018 18388 10 Skin 0.20 protein_coding synonymous_variant LOW 666C>T Tyr222Tyr
M0107963 DMKGBHCF_00018 18446 11 Skin 0.22 protein_coding missense_variant MODERATE 608T>C Val203Ala
M0107964 DMKGBHCF_00018 18449 11 Skin 0.22 protein_coding missense_variant MODERATE 605C>T Ala202Val
M0107965 DMKGBHCF_00018 18451 11 Skin 0.22 protein_coding missense_variant MODERATE 603C>A His201Gln
M0107966 DMKGBHCF_00019 19301 3 Skin 0.06 protein_coding synonymous_variant LOW 577T>C Leu193Leu
M0107967 DMKGBHCF_00019 19554 3 Skin 0.06 protein_coding synonymous_variant LOW 324T>G Leu108Leu
M0107968 DMKGBHCF_00019 19864 14 Skin 0.29 protein_coding missense_variant MODERATE 14A>C Gln5Pro
M0107969 DMKGBHCF_00014 19958 12 Skin 0.24 protein_coding upstream_gene_variant MODIFIER -4598A>G None
M0107970 DMKGBHCF_00014 19966 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4606C>A None
M0107971 DMKGBHCF_00014 19985 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4625T>C None
M0107972 DMKGBHCF_00015 16022 4 Skin 0.08 protein_coding missense_variant MODERATE 320A>G Asn107Ser
M0107973 DMKGBHCF_00015 16083 3 Skin 0.06 protein_coding missense_variant MODERATE 259C>A Arg87Ser
M0107974 DMKGBHCF_00015 16087 3 Skin 0.06 protein_coding synonymous_variant LOW 255C>A Gly85Gly
M0107975 DMKGBHCF_00015 16159 3 Skin 0.06 protein_coding synonymous_variant LOW 183G>T Arg61Arg
M0107976 DMKGBHCF_00016 16860 8 Skin 0.16 protein_coding missense_variant MODERATE 486T>G Asp162Glu
M0107977 DMKGBHCF_00017 17570 11 Skin 0.22 protein_coding synonymous_variant LOW 612G>T Thr204Thr
M0107978 DMKGBHCF_00018 18235 10 Skin 0.20 protein_coding synonymous_variant LOW 819C>T Ile273Ile
M0107979 DMKGBHCF_00018 18280 9 Skin 0.18 protein_coding synonymous_variant LOW 774G>A Lys258Lys
M0107980 DMKGBHCF_00018 18591 9 Skin 0.18 protein_coding missense_variant MODERATE 463G>A Ala155Thr
M0107981 DMKGBHCF_00018 18913 6 Skin 0.12 protein_coding synonymous_variant LOW 141T>A Arg47Arg
M0107982 DMKGBHCF_00018 18924 6 Skin 0.12 protein_coding synonymous_variant LOW 130C>T Leu44Leu
M0107983 DMKGBHCF_00018 18928 6 Skin 0.12 protein_coding synonymous_variant LOW 126C>G Ser42Ser
M0107984 DMKGBHCF_00018 18952 6 Skin 0.12 protein_coding synonymous_variant LOW 102C>G Ala34Ala
M0107985 DMKGBHCF_00018 18990 5 Skin 0.10 protein_coding missense_variant MODERATE 64C>G Leu22Val
M0107986 DMKGBHCF_00018 18999 5 Skin 0.10 protein_coding synonymous_variant LOW 55T>C Leu19Leu
M0107987 DMKGBHCF_00018 19021 5 Skin 0.10 protein_coding synonymous_variant LOW 33C>T Cys11Cys
M0107988 DMKGBHCF_00018 19024 5 Skin 0.10 protein_coding synonymous_variant LOW 30T>C Thr10Thr
M0107989 DMKGBHCF_00018 19036 5 Skin 0.10 protein_coding synonymous_variant LOW 18G>T Ala6Ala
M0107990 DMKGBHCF_00019 19075 5 Skin 0.10 protein_coding missense_variant MODERATE 803A>C Glu268Ala
M0107991 DMKGBHCF_00019 19089 5 Skin 0.10 protein_coding synonymous_variant LOW 789A>C Pro263Pro
M0107992 DMKGBHCF_00019 19091 5 Skin 0.10 protein_coding missense_variant MODERATE 787C>A Pro263Thr
M0107993 DMKGBHCF_00019 19105 5 Skin 0.10 protein_coding missense_variant MODERATE 773A>C Asn258Thr
M0107994 DMKGBHCF_00019 19107 5 Skin 0.10 protein_coding synonymous_variant LOW 771G>A Ala257Ala
M0107995 DMKGBHCF_00019 19818 3 Skin 0.06 protein_coding synonymous_variant LOW 60T>C Tyr20Tyr
M0107996 DMKGBHCF_00017 18073 7 Skin 0.14 protein_coding missense_variant MODERATE 109T>C Phe37Leu
M0107997 DMKGBHCF_00017 18080 7 Skin 0.14 protein_coding synonymous_variant LOW 102G>A Ala34Ala
M0107998 DMKGBHCF_00018 18493 11 Skin 0.22 protein_coding synonymous_variant LOW 561C>A Gly187Gly
M0107999 DMKGBHCF_00018 18567 8 Skin 0.16 protein_coding synonymous_variant LOW 487T>C Leu163Leu
M0108000 DMKGBHCF_00018 18568 8 Skin 0.16 protein_coding synonymous_variant LOW 486T>G Gly162Gly
M0108001 DMKGBHCF_00018 18569 8 Skin 0.16 protein_coding missense_variant MODERATE 485G>A Gly162Asp
M0108002 DMKGBHCF_00018 18603 8 Skin 0.16 protein_coding synonymous_variant LOW 451T>C Leu151Leu
M0108003 DMKGBHCF_00105 92435 3 Skin 0.06 protein_coding stop_lost&splice_region_variant HIGH 286T>G Ter96Gluext*?
M0108004 DMKGBHCF_00015 15724 6 Skin 0.12 protein_coding synonymous_variant LOW 618A>G Glu206Glu
M0108005 DMKGBHCF_00016 16873 6 Skin 0.12 protein_coding missense_variant MODERATE 473T>A Leu158Gln
M0108006 DMKGBHCF_00016 16908 5 Skin 0.10 protein_coding synonymous_variant LOW 438T>G Ala146Ala
M0108007 DMKGBHCF_00016 16958 5 Skin 0.10 protein_coding missense_variant MODERATE 388A>G Lys130Glu
M0108008 DMKGBHCF_00016 16986 3 Skin 0.06 protein_coding synonymous_variant LOW 360G>C Arg120Arg
M0108009 DMKGBHCF_00015 16037 6 Skin 0.12 protein_coding missense_variant MODERATE 305A>T Gln102Leu
M0108010 DMKGBHCF_00018 18700 7 Skin 0.14 protein_coding synonymous_variant LOW 354G>A Ser118Ser
M0108011 DMKGBHCF_00015 15664 7 Skin 0.14 protein_coding synonymous_variant LOW 678G>C Val226Val
M0108012 DMKGBHCF_00017 18032 3 Skin 0.06 protein_coding synonymous_variant LOW 150C>G Leu50Leu
M0108013 DMKGBHCF_00016 16703 5 Skin 0.10 protein_coding missense_variant MODERATE 643C>G Gln215Glu
M0108014 DMKGBHCF_00016 16704 5 Skin 0.10 protein_coding synonymous_variant LOW 642C>T Thr214Thr
M0108015 DMKGBHCF_00016 17136 3 Skin 0.06 protein_coding synonymous_variant LOW 210A>G Val70Val
M0108016 DMKGBHCF_00016 17148 3 Skin 0.06 protein_coding synonymous_variant LOW 198T>C Pro66Pro
M0108017 DMKGBHCF_00016 17160 3 Skin 0.06 protein_coding synonymous_variant LOW 186T>C Leu62Leu
M0108018 DMKGBHCF_00016 17166 3 Skin 0.06 protein_coding missense_variant MODERATE 180C>A Asp60Glu
M0108019 DMKGBHCF_00019 19656 4 Skin 0.08 protein_coding synonymous_variant LOW 222A>G Glu74Glu
M0108020 DMKGBHCF_00019 19665 3 Skin 0.06 protein_coding synonymous_variant LOW 213A>G Glu71Glu
M0108021 DMKGBHCF_00019 19685 3 Skin 0.06 protein_coding missense_variant MODERATE 193G>C Gly65Arg
M0108022 DMKGBHCF_00019 19710 3 Skin 0.06 protein_coding synonymous_variant LOW 168T>C Ser56Ser
M0108023 DMKGBHCF_00014 19950 9 Skin 0.18 protein_coding upstream_gene_variant MODIFIER -4590T>G None
M0108024 DMKGBHCF_00014 19917 5 Skin 0.10 protein_coding upstream_gene_variant MODIFIER -4557C>G None
M0108025 DMKGBHCF_00016 17243 4 Skin 0.08 protein_coding missense_variant MODERATE 103C>A Pro35Thr
M0108026 DMKGBHCF_00016 17257 3 Skin 0.06 protein_coding missense_variant MODERATE 89C>G Thr30Ser
M0108027 DMKGBHCF_00084 82935 4 Skin 0.08 protein_coding upstream_gene_variant MODIFIER -4593C>T None
M0108028 DMKGBHCF_00084 82939 4 Skin 0.08 protein_coding upstream_gene_variant MODIFIER -4597G>A None
M0108029 DMKGBHCF_00084 82987 4 Skin 0.08 protein_coding upstream_gene_variant MODIFIER -4645A>C None
M0108030 DMKGBHCF_00017 17900 6 Skin 0.12 protein_coding synonymous_variant LOW 282T>C Gly94Gly
M0108031 DMKGBHCF_00017 17920 6 Skin 0.12 protein_coding synonymous_variant LOW 262T>C Leu88Leu
M0108032 DMKGBHCF_00017 17921 6 Skin 0.12 protein_coding synonymous_variant LOW 261C>G Thr87Thr
M0108033 DMKGBHCF_00017 17933 6 Skin 0.12 protein_coding synonymous_variant LOW 249C>G Pro83Pro
M0108034 DMKGBHCF_00017 17698 3 Skin 0.06 protein_coding missense_variant MODERATE 484G>A Val162Ile






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group
DMKGBHCF_00077 Chromium (Cr) 99.8 9.7e-229 1 416 1.0000 1.0000 experiment
DMKGBHCF_00077 Chromium (Cr) 100 3.4e-227 1 416 1.0000 1.0000 prediction
DMKGBHCF_00078 Chromium (Cr), Methyl Viologen [class: Paraquat], Menadione [class: Naphthoquinone] 100 2.9e-178 1 314 1.0000 1.0000 prediction
DMKGBHCF_00086 Mercury (Hg) 96.7 9.7e-42 1 91 1.0000 1.0000 prediction






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
DMKGBHCF_00022 PHI:3165 hopA1 83.6 6.6e-140 2 281 0.9964 0.9556 eudicots leaf spot (celery); leaf spot (eggplant); leaf spot (sweet pepper); wilt rot (muskmelon); stem rot (muskmelon); stem rot (chrysanthemum); leaf spot (soybean) effector (plant avirulence determinant) effector (plant avirulence determinant)






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
DMKGBHCF_00077 2.A.51.1.3 99.8 3.7e-227 1 416 1.0000 1.0000 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.51 The Chromate Ion Transporter (CHR) Family
DMKGBHCF_00176 2.A.53.3.1 74.7 8e-205 1 494 0.9980 0.9939 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.53 The Sulfate Permease (SulP) Family