Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C1122
  Reference Plasmid   NZ_CP118011.1
  Reference Plasmid Size   54979
  Reference Plasmid GC Content   0.27
  Reference Plasmid Mobility Type   mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0197005 MBDMOHFA_00012 11896 3 Gut 0.75 protein_coding synonymous_variant LOW 549A>T Thr183Thr
M0197006 MBDMOHFA_00012 11916 3 Gut 0.75 protein_coding synonymous_variant LOW 529C>T Leu177Leu
M0197007 MBDMOHFA_00012 11975 3 Gut 0.75 protein_coding missense_variant MODERATE 470C>G Thr157Ser
M0197008 MBDMOHFA_00012 12061 3 Gut 0.75 protein_coding synonymous_variant LOW 384G>A Glu128Glu
M0197009 MBDMOHFA_00012 12124 3 Gut 0.75 protein_coding synonymous_variant LOW 321A>C Thr107Thr
M0197010 MBDMOHFA_00012 12160 3 Gut 0.75 protein_coding synonymous_variant LOW 285G>A Thr95Thr
M0197011 MBDMOHFA_00012 12163 3 Gut 0.75 protein_coding synonymous_variant LOW 282T>A Thr94Thr
M0197012 MBDMOHFA_00012 12169 3 Gut 0.75 protein_coding synonymous_variant LOW 276T>C Leu92Leu
M0197013 MBDMOHFA_00012 12177 3 Gut 0.75 protein_coding synonymous_variant LOW 268C>T Leu90Leu
M0197014 MBDMOHFA_00012 12210 3 Gut 0.75 protein_coding missense_variant MODERATE 235T>C Tyr79His
M0197015 MBDMOHFA_00012 12220 3 Gut 0.75 protein_coding synonymous_variant LOW 225A>G Lys75Lys
M0197016 MBDMOHFA_00012 12223 3 Gut 0.75 protein_coding synonymous_variant LOW 222C>G Leu74Leu
M0197017 MBDMOHFA_00012 12253 3 Gut 0.75 protein_coding synonymous_variant LOW 192C>T Asp64Asp
M0197018 MBDMOHFA_00012 12280 3 Gut 0.75 protein_coding synonymous_variant LOW 165T>G Gly55Gly
M0197019 MBDMOHFA_00012 12283 3 Gut 0.75 protein_coding synonymous_variant LOW 162A>T Ser54Ser
M0197020 MBDMOHFA_00012 12289 3 Gut 0.75 protein_coding synonymous_variant LOW 156T>A Ile52Ile
M0197021 MBDMOHFA_00012 12294 3 Gut 0.75 protein_coding missense_variant MODERATE 151G>A Ala51Thr
M0197022 MBDMOHFA_00012 12304 3 Gut 0.75 protein_coding synonymous_variant LOW 141A>G Lys47Lys
M0197023 MBDMOHFA_00012 12307 3 Gut 0.75 protein_coding missense_variant MODERATE 138G>T Met46Ile
M0197024 MBDMOHFA_00012 12308 3 Gut 0.75 protein_coding missense_variant MODERATE 137T>C Met46Thr
M0197025 MBDMOHFA_00012 12316 3 Gut 0.75 protein_coding synonymous_variant LOW 129C>T Pro43Pro
M0197026 MBDMOHFA_00012 12319 3 Gut 0.75 protein_coding synonymous_variant LOW 126G>A Arg42Arg
M0197027 MBDMOHFA_00012 12325 3 Gut 0.75 protein_coding synonymous_variant LOW 120T>C Phe40Phe
M0197028 MBDMOHFA_00012 12359 3 Gut 0.75 protein_coding missense_variant MODERATE 86A>G Lys29Arg
M0197029 MBDMOHFA_00012 12367 3 Gut 0.75 protein_coding synonymous_variant LOW 78A>C Gly26Gly
M0197030 MBDMOHFA_00012 12382 3 Gut 0.75 protein_coding synonymous_variant LOW 63T>C Ser21Ser
M0197031 MBDMOHFA_00012 12387 3 Gut 0.75 protein_coding missense_variant MODERATE 58G>A Glu20Lys
M0197032 MBDMOHFA_00012 12409 3 Gut 0.75 protein_coding synonymous_variant LOW 36A>G Glu12Glu
M0197033 MBDMOHFA_00012 12430 3 Gut 0.75 protein_coding synonymous_variant LOW 15T>C Tyr5Tyr
M0197034 MBDMOHFA_00012 12471 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -27G>A None
M0197035 MBDMOHFA_00012 12493 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -49T>C None
M0197036 MBDMOHFA_00012 12513 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -69G>A None
M0197037 MBDMOHFA_00012 12541 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -97T>A None
M0197038 MBDMOHFA_00012 12545 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -101A>T None
M0197039 MBDMOHFA_00012 12546 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -102T>A None
M0197040 MBDMOHFA_00012 12548 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -104A>C None
M0197041 MBDMOHFA_00012 12603 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -159A>C None
M0197042 MBDMOHFA_00012 12608 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -164C>T None
M0197043 MBDMOHFA_00012 12609 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -165A>C None
M0197044 MBDMOHFA_00012 12610 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -166G>T None
M0197045 MBDMOHFA_00012 12619 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -175T>C None
M0197046 MBDMOHFA_00013 12786 3 Gut 0.75 protein_coding missense_variant MODERATE 122T>C Ile41Thr
M0197047 MBDMOHFA_00013 12823 3 Gut 0.75 protein_coding synonymous_variant LOW 159A>T Gly53Gly






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
MBDMOHFA_00002 ABF47318.1|GH73 99 5.05e-219 1 297 1 0.8892





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
MBDMOHFA_00002 3.A.7.14.2 98.3 9.7e-174 1 297 1.0000 0.9834 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
MBDMOHFA_00003 3.A.7.14.2 94.5 0 1 837 1.0000 2.7517 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
MBDMOHFA_00005 3.A.7.14.2 70.3 2.6e-95 1 252 1.0000 0.8543 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
MBDMOHFA_00061 3.A.7.14.2 94.4 2.9e-129 16 266 0.9436 0.8311 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
MBDMOHFA_00064 3.A.7.14.2 93.4 2.6e-158 1 302 1.0000 1.0000 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
MBDMOHFA_00066 3.A.7.14.2 98.9 6.9e-198 1 359 1.0000 1.1887 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
MBDMOHFA_00069 3.A.7.14.2 99 0 1 716 0.9931 2.3709 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family