Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C1132
  Reference Plasmid   NZ_CP119189.1
  Reference Plasmid Size   224659
  Reference Plasmid GC Content   0.27
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0197074 FKDNOJHF_00047 48804 16 Gut 0.22 protein_coding upstream_gene_variant MODIFIER -1075A>G None
M0197075 FKDNOJHF_00049 49065 16 Gut 0.22 protein_coding missense_variant MODERATE 107T>C Val36Ala
M0197076 FKDNOJHF_00050 49689 14 Gut 0.19 protein_coding synonymous_variant LOW 267A>G Val89Val
M0197077 FKDNOJHF_00050 49708 4 Gut 0.06 protein_coding synonymous_variant LOW 286A>C Arg96Arg
M0197078 FKDNOJHF_00050 49740 4 Gut 0.06 protein_coding synonymous_variant LOW 318T>G Gly106Gly
M0197079 FKDNOJHF_00050 49803 3 Gut 0.04 protein_coding synonymous_variant LOW 381T>C Asn127Asn
M0197080 FKDNOJHF_00050 49815 3 Gut 0.04 protein_coding synonymous_variant LOW 393T>C Ile131Ile
M0197081 FKDNOJHF_00050 49990 28 Gut 0.39 protein_coding missense_variant MODERATE 568A>G Ile190Val
M0197082 FKDNOJHF_00051 50140 28 Gut 0.39 protein_coding synonymous_variant LOW 123C>T Asp41Asp
M0197083 FKDNOJHF_00055 53537 14 Gut 0.19 protein_coding upstream_gene_variant MODIFIER -81G>A None
M0197084 FKDNOJHF_00055 54391 3 Gut 0.04 protein_coding synonymous_variant LOW 774T>C Phe258Phe
M0197085 FKDNOJHF_00055 54716 17 Gut 0.24 protein_coding missense_variant MODERATE 1099T>C Phe367Leu
M0197086 FKDNOJHF_00047 49318 10 Gut 0.14 protein_coding upstream_gene_variant MODIFIER -1589T>A None
M0197087 FKDNOJHF_00050 49674 10 Gut 0.14 protein_coding synonymous_variant LOW 252T>C Ala84Ala
M0197088 FKDNOJHF_00050 49677 10 Gut 0.14 protein_coding synonymous_variant LOW 255C>A Val85Val
M0197089 FKDNOJHF_00050 49683 10 Gut 0.14 protein_coding synonymous_variant LOW 261C>T Ser87Ser
M0197090 FKDNOJHF_00050 49701 10 Gut 0.14 protein_coding synonymous_variant LOW 279C>T Gly93Gly
M0197091 FKDNOJHF_00049 48989 15 Gut 0.21 protein_coding missense_variant MODERATE 31G>A Val11Ile
M0197092 FKDNOJHF_00051 50377 3 Gut 0.04 protein_coding synonymous_variant LOW 360G>A Thr120Thr
M0197093 FKDNOJHF_00051 50401 3 Gut 0.04 protein_coding synonymous_variant LOW 384T>C Phe128Phe
M0197094 FKDNOJHF_00052 50806 4 Gut 0.06 protein_coding missense_variant MODERATE 23G>C Gly8Ala
M0197095 FKDNOJHF_00052 50813 4 Gut 0.06 protein_coding missense_variant MODERATE 30G>A Met10Ile
M0197096 FKDNOJHF_00047 49229 4 Gut 0.06 protein_coding upstream_gene_variant MODIFIER -1500G>T None
M0197097 FKDNOJHF_00054 53150 5 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -21G>A None
M0197098 FKDNOJHF_00053 51644 3 Gut 0.04 protein_coding missense_variant MODERATE 103C>T Pro35Ser
M0197099 FKDNOJHF_00053 51781 3 Gut 0.04 protein_coding synonymous_variant LOW 240T>C Leu80Leu
M0197100 FKDNOJHF_00053 51817 3 Gut 0.04 protein_coding synonymous_variant LOW 276G>A Gly92Gly
M0197101 FKDNOJHF_00053 51874 3 Gut 0.04 protein_coding synonymous_variant LOW 333T>C Phe111Phe
M0197102 FKDNOJHF_00053 52201 3 Gut 0.04 protein_coding synonymous_variant LOW 660G>A Thr220Thr
M0197103 FKDNOJHF_00053 52203 3 Gut 0.04 protein_coding missense_variant MODERATE 662A>T Glu221Val
M0197104 FKDNOJHF_00053 52246 3 Gut 0.04 protein_coding synonymous_variant LOW 705T>C Arg235Arg
M0197105 FKDNOJHF_00053 52258 3 Gut 0.04 protein_coding synonymous_variant LOW 717T>C Ser239Ser
M0197106 FKDNOJHF_00047 52308 3 Gut 0.04 protein_coding upstream_gene_variant MODIFIER -4579T>C None
M0197107 FKDNOJHF_00047 52432 3 Gut 0.04 protein_coding upstream_gene_variant MODIFIER -4703A>G None
M0197108 FKDNOJHF_00047 52612 3 Gut 0.04 protein_coding upstream_gene_variant MODIFIER -4883C>T None
M0197109 FKDNOJHF_00054 52751 3 Gut 0.04 protein_coding upstream_gene_variant MODIFIER -420T>C None
M0197110 FKDNOJHF_00054 52768 3 Gut 0.04 protein_coding upstream_gene_variant MODIFIER -403C>T None
M0197111 FKDNOJHF_00054 52797 3 Gut 0.04 protein_coding upstream_gene_variant MODIFIER -374G>A None
M0197112 FKDNOJHF_00054 52827 3 Gut 0.04 protein_coding upstream_gene_variant MODIFIER -344A>C None
M0197113 FKDNOJHF_00054 52828 3 Gut 0.04 protein_coding upstream_gene_variant MODIFIER -343A>T None
M0197114 FKDNOJHF_00054 52843 3 Gut 0.04 protein_coding upstream_gene_variant MODIFIER -328C>T None
M0197115 FKDNOJHF_00054 52926 14 Gut 0.19 protein_coding upstream_gene_variant MODIFIER -245C>T None
M0197116 FKDNOJHF_00055 54717 15 Gut 0.21 protein_coding missense_variant MODERATE 1100T>C Phe367Ser
M0197117 FKDNOJHF_00047 49253 3 Gut 0.04 protein_coding upstream_gene_variant MODIFIER -1524C>A None
M0197118 FKDNOJHF_00047 49256 3 Gut 0.04 protein_coding upstream_gene_variant MODIFIER -1527T>C None
M0197119 FKDNOJHF_00047 49257 3 Gut 0.04 protein_coding upstream_gene_variant MODIFIER -1528T>G None
M0197120 FKDNOJHF_00047 49264 3 Gut 0.04 protein_coding upstream_gene_variant MODIFIER -1535T>G None
M0197121 FKDNOJHF_00047 49266 3 Gut 0.04 protein_coding upstream_gene_variant MODIFIER -1537T>C None
M0197122 FKDNOJHF_00055 53468 12 Gut 0.17 protein_coding upstream_gene_variant MODIFIER -150A>G None
M0197123 FKDNOJHF_00055 53492 11 Gut 0.15 protein_coding upstream_gene_variant MODIFIER -126G>A None
M0197124 FKDNOJHF_00055 53499 10 Gut 0.14 protein_coding upstream_gene_variant MODIFIER -119A>C None
M0197125 FKDNOJHF_00055 53564 9 Gut 0.13 protein_coding upstream_gene_variant MODIFIER -54A>G None
M0197126 FKDNOJHF_00055 53680 17 Gut 0.24 protein_coding synonymous_variant LOW 63A>G Thr21Thr
M0197127 FKDNOJHF_00055 53831 17 Gut 0.24 protein_coding missense_variant MODERATE 214A>G Lys72Glu
M0197128 FKDNOJHF_00055 53854 8 Gut 0.11 protein_coding synonymous_variant LOW 237C>T His79His
M0197129 FKDNOJHF_00055 53950 15 Gut 0.21 protein_coding synonymous_variant LOW 333T>C His111His
M0197130 FKDNOJHF_00055 53978 14 Gut 0.19 protein_coding missense_variant MODERATE 361G>A Asp121Asn
M0197131 FKDNOJHF_00055 54020 13 Gut 0.18 protein_coding synonymous_variant LOW 403C>T Leu135Leu
M0197132 FKDNOJHF_00055 54031 9 Gut 0.13 protein_coding synonymous_variant LOW 414G>A Arg138Arg
M0197133 FKDNOJHF_00055 54034 9 Gut 0.13 protein_coding synonymous_variant LOW 417C>T Cys139Cys
M0197134 FKDNOJHF_00055 54040 11 Gut 0.15 protein_coding synonymous_variant LOW 423G>A Glu141Glu
M0197135 FKDNOJHF_00055 54085 14 Gut 0.19 protein_coding synonymous_variant LOW 468C>A Thr156Thr
M0197136 FKDNOJHF_00055 54172 10 Gut 0.14 protein_coding synonymous_variant LOW 555A>G Lys185Lys
M0197137 FKDNOJHF_00055 54208 10 Gut 0.14 protein_coding synonymous_variant LOW 591C>T Asp197Asp
M0197138 FKDNOJHF_00055 54237 15 Gut 0.21 protein_coding missense_variant MODERATE 620A>C Asn207Thr
M0197139 FKDNOJHF_00055 54248 14 Gut 0.19 protein_coding missense_variant MODERATE 631A>C Met211Leu
M0197140 FKDNOJHF_00055 54271 14 Gut 0.19 protein_coding synonymous_variant LOW 654C>G Gly218Gly
M0197141 FKDNOJHF_00055 54274 14 Gut 0.19 protein_coding synonymous_variant LOW 657A>G Val219Val
M0197142 FKDNOJHF_00055 54403 13 Gut 0.18 protein_coding synonymous_variant LOW 786C>T Ser262Ser
M0197143 FKDNOJHF_00054 53260 9 Gut 0.13 protein_coding synonymous_variant LOW 90T>C Asn30Asn
M0197144 FKDNOJHF_00055 53361 11 Gut 0.15 protein_coding upstream_gene_variant MODIFIER -257C>T None
M0197145 FKDNOJHF_00055 53372 11 Gut 0.15 protein_coding upstream_gene_variant MODIFIER -246G>A None
M0197146 FKDNOJHF_00055 53397 12 Gut 0.17 protein_coding upstream_gene_variant MODIFIER -221C>T None
M0197147 FKDNOJHF_00055 53399 12 Gut 0.17 protein_coding upstream_gene_variant MODIFIER -219A>G None
M0197148 FKDNOJHF_00055 53432 8 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -186G>A None
M0197149 FKDNOJHF_00055 53438 10 Gut 0.14 protein_coding upstream_gene_variant MODIFIER -180A>G None
M0197150 FKDNOJHF_00054 53110 7 Gut 0.10 protein_coding upstream_gene_variant MODIFIER -61A>C None
M0197151 FKDNOJHF_00054 53112 7 Gut 0.10 protein_coding upstream_gene_variant MODIFIER -59A>G None
M0197152 FKDNOJHF_00054 53113 7 Gut 0.10 protein_coding upstream_gene_variant MODIFIER -58C>A None
M0197153 FKDNOJHF_00054 53120 7 Gut 0.10 protein_coding upstream_gene_variant MODIFIER -51G>A None
M0197154 FKDNOJHF_00054 53133 7 Gut 0.10 protein_coding upstream_gene_variant MODIFIER -38A>G None
M0197155 FKDNOJHF_00054 53182 8 Gut 0.11 protein_coding missense_variant MODERATE 12T>A Asp4Glu
M0197156 FKDNOJHF_00054 53303 8 Gut 0.11 protein_coding synonymous_variant LOW 133C>T Leu45Leu
M0197157 FKDNOJHF_00047 51119 3 Gut 0.04 protein_coding upstream_gene_variant MODIFIER -3390G>A None
M0197158 FKDNOJHF_00055 53785 5 Gut 0.07 protein_coding synonymous_variant LOW 168T>C Asp56Asp
M0197159 FKDNOJHF_00055 53713 3 Gut 0.04 protein_coding synonymous_variant LOW 96C>T Ser32Ser
M0197160 FKDNOJHF_00055 53737 4 Gut 0.06 protein_coding synonymous_variant LOW 120G>A Thr40Thr
M0197161 FKDNOJHF_00055 53782 3 Gut 0.04 protein_coding synonymous_variant LOW 165G>A Thr55Thr
M0197162 FKDNOJHF_00055 53812 3 Gut 0.04 protein_coding synonymous_variant LOW 195A>G Gln65Gln
M0197163 FKDNOJHF_00055 53864 3 Gut 0.04 protein_coding missense_variant MODERATE 247G>A Val83Ile
M0197164 FKDNOJHF_00055 53875 3 Gut 0.04 protein_coding synonymous_variant LOW 258A>G Ala86Ala
M0197165 FKDNOJHF_00055 53944 3 Gut 0.04 protein_coding synonymous_variant LOW 327C>T His109His
M0197166 FKDNOJHF_00055 53956 3 Gut 0.04 protein_coding synonymous_variant LOW 339T>A Ile113Ile
M0197167 FKDNOJHF_00055 54196 3 Gut 0.04 protein_coding synonymous_variant LOW 579T>G Leu193Leu
M0197168 FKDNOJHF_00055 54202 3 Gut 0.04 protein_coding synonymous_variant LOW 585G>A Val195Val






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
FKDNOJHF_00070 ARO:3000593 100 1.24e-189 1 257 1.0000 1.0000 macrolide antibiotic Erm 23S ribosomal RNA methyltransferase antibiotic target alteration
FKDNOJHF_00073 ARO:3000375 98.8 9.7e-175 1 245 1.0000 0.9879 macrolide antibiotic Erm 23S ribosomal RNA methyltransferase antibiotic target alteration
FKDNOJHF_00078 ARO:3002597 100 0 1 479 1.0000 1.0000 aminoglycoside antibiotic aminoglycoside bifunctional resistance protein antibiotic inactivation
FKDNOJHF_00131 ARO:3000593 100 1.24e-189 1 257 1.0000 1.0000 macrolide antibiotic Erm 23S ribosomal RNA methyltransferase antibiotic target alteration
FKDNOJHF_00134 ARO:3000375 98.8 9.7e-175 1 245 1.0000 0.9879 macrolide antibiotic Erm 23S ribosomal RNA methyltransferase antibiotic target alteration
FKDNOJHF_00139 ARO:3002597 100 0 1 479 1.0000 1.0000 aminoglycoside antibiotic aminoglycoside bifunctional resistance protein antibiotic inactivation
FKDNOJHF_00191 ARO:3004601 100 1.54e-129 1 166 1.0000 1.0000 lincosamide antibiotic lincosamide nucleotidyltransferase (LNU) antibiotic inactivation
FKDNOJHF_00229 ARO:3000347 79.3 2.96e-133 1 242 0.9959 0.9959 macrolide antibiotic Erm 23S ribosomal RNA methyltransferase antibiotic target alteration
FKDNOJHF_00231 ARO:3003746 99.8 0 1 637 1.0000 0.9725 oxazolidinone antibiotic Miscellaneous ABC-F subfamily ATP-binding cassette ribosomal protection proteins antibiotic target protection
FKDNOJHF_00234 ARO:3002704 98.9 0 1 475 1.0000 1.0000 phenicol antibiotic major facilitator superfamily (MFS) antibiotic efflux pump antibiotic efflux






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
FKDNOJHF_00076 CCO10203.2|GH1 86.9 1.56e-244 1 390 1 1
FKDNOJHF_00079 CCO10203.2|GH1 86.9 1.56e-244 1 390 1 1
FKDNOJHF_00137 CCO10203.2|GH1 86.9 1.56e-244 1 390 1 1
FKDNOJHF_00140 CCO10203.2|GH1 86.9 1.56e-244 1 390 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
FKDNOJHF_00231 3.A.1.211.25 99.5 0 1 637 1.0000 0.9969 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily