Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C1153
  Reference Plasmid   NZ_CP122705.1
  Reference Plasmid Size   135522
  Reference Plasmid GC Content   0.50
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0198022 FOMKPHKJ_00081 70992 4 Gut 0.57 protein_coding upstream_gene_variant MODIFIER -3774A>C None
M0198023 FOMKPHKJ_00085 71428 4 Gut 0.57 protein_coding missense_variant MODERATE 1023A>C Arg341Ser
M0198024 FOMKPHKJ_00085 71568 4 Gut 0.57 protein_coding missense_variant MODERATE 883G>A Glu295Lys
M0198025 FOMKPHKJ_00085 71587 4 Gut 0.57 protein_coding synonymous_variant LOW 864C>T Val288Val
M0198026 FOMKPHKJ_00085 71617 4 Gut 0.57 protein_coding synonymous_variant LOW 834C>T Ala278Ala
M0198027 FOMKPHKJ_00085 71653 4 Gut 0.57 protein_coding missense_variant MODERATE 798A>T Lys266Asn
M0198028 FOMKPHKJ_00085 71662 4 Gut 0.57 protein_coding missense_variant MODERATE 789G>A Met263Ile
M0198029 FOMKPHKJ_00085 71693 4 Gut 0.57 protein_coding missense_variant MODERATE 758G>A Gly253Glu
M0198030 FOMKPHKJ_00085 71713 4 Gut 0.57 protein_coding synonymous_variant LOW 738C>T Asp246Asp
M0198031 FOMKPHKJ_00086 72541 4 Gut 0.57 protein_coding synonymous_variant LOW 474T>C Gly158Gly
M0198032 FOMKPHKJ_00086 72544 4 Gut 0.57 protein_coding synonymous_variant LOW 471T>A Pro157Pro
M0198033 FOMKPHKJ_00086 72555 4 Gut 0.57 protein_coding missense_variant MODERATE 460G>A Val154Ile
M0198034 FOMKPHKJ_00086 72564 4 Gut 0.57 protein_coding missense_variant MODERATE 451A>G Lys151Glu
M0198035 FOMKPHKJ_00086 72567 4 Gut 0.57 protein_coding missense_variant MODERATE 448A>G Ser150Gly
M0198036 FOMKPHKJ_00086 72594 4 Gut 0.57 protein_coding missense_variant MODERATE 421A>C Ser141Arg
M0198037 FOMKPHKJ_00086 72607 4 Gut 0.57 protein_coding synonymous_variant LOW 408A>T Gly136Gly
M0198038 FOMKPHKJ_00086 72614 4 Gut 0.57 protein_coding missense_variant MODERATE 401G>A Gly134Asp
M0198039 FOMKPHKJ_00086 72642 4 Gut 0.57 protein_coding missense_variant MODERATE 373G>A Glu125Lys
M0198040 FOMKPHKJ_00086 72712 4 Gut 0.57 protein_coding synonymous_variant LOW 303A>G Lys101Lys
M0198041 FOMKPHKJ_00086 72775 4 Gut 0.57 protein_coding synonymous_variant LOW 240T>C Asn80Asn
M0198042 FOMKPHKJ_00086 72794 4 Gut 0.57 protein_coding missense_variant MODERATE 221T>C Ile74Thr
M0198043 FOMKPHKJ_00086 72823 4 Gut 0.57 protein_coding missense_variant MODERATE 192C>A Asn64Lys
M0198044 FOMKPHKJ_00086 72824 4 Gut 0.57 protein_coding missense_variant MODERATE 191A>G Asn64Ser
M0198045 FOMKPHKJ_00086 72842 4 Gut 0.57 protein_coding missense_variant MODERATE 173C>T Ser58Phe
M0198046 FOMKPHKJ_00086 72845 4 Gut 0.57 protein_coding missense_variant MODERATE 170T>C Val57Ala
M0198047 FOMKPHKJ_00086 72849 4 Gut 0.57 protein_coding missense_variant MODERATE 166C>A Gln56Lys
M0198048 FOMKPHKJ_00086 72892 4 Gut 0.57 protein_coding synonymous_variant LOW 123T>C Cys41Cys
M0198049 FOMKPHKJ_00086 72898 4 Gut 0.57 protein_coding synonymous_variant LOW 117T>A Pro39Pro
M0198050 FOMKPHKJ_00086 72905 4 Gut 0.57 protein_coding missense_variant MODERATE 110A>C Glu37Ala
M0198051 FOMKPHKJ_00086 72917 4 Gut 0.57 protein_coding missense_variant MODERATE 98C>T Thr33Ile
M0198052 FOMKPHKJ_00086 72982 4 Gut 0.57 protein_coding synonymous_variant LOW 33A>G Leu11Leu
M0198053 FOMKPHKJ_00086 73000 4 Gut 0.57 protein_coding missense_variant MODERATE 15G>T Leu5Phe
M0198054 FOMKPHKJ_00083 73076 4 Gut 0.57 protein_coding upstream_gene_variant MODIFIER -3216G>A None
M0198055 FOMKPHKJ_00083 73079 4 Gut 0.57 protein_coding upstream_gene_variant MODIFIER -3219G>T None
M0198056 FOMKPHKJ_00087 73092 4 Gut 0.57 protein_coding missense_variant MODERATE 676G>A Val226Met
M0198057 FOMKPHKJ_00087 73113 4 Gut 0.57 protein_coding missense_variant MODERATE 655C>G Leu219Val
M0198058 FOMKPHKJ_00087 73114 4 Gut 0.57 protein_coding synonymous_variant LOW 654C>T Gly218Gly
M0198059 FOMKPHKJ_00087 73176 4 Gut 0.57 protein_coding synonymous_variant LOW 592T>C Leu198Leu
M0198060 FOMKPHKJ_00087 73179 4 Gut 0.57 protein_coding missense_variant MODERATE 589G>C Glu197Gln
M0198061 FOMKPHKJ_00087 73181 4 Gut 0.57 protein_coding missense_variant MODERATE 587C>T Ala196Val
M0198062 FOMKPHKJ_00087 73221 4 Gut 0.57 protein_coding missense_variant MODERATE 547G>A Gly183Arg
M0198063 FOMKPHKJ_00087 73225 4 Gut 0.57 protein_coding synonymous_variant LOW 543G>A Gln181Gln
M0198064 FOMKPHKJ_00087 73228 4 Gut 0.57 protein_coding synonymous_variant LOW 540C>A Ala180Ala
M0198065 FOMKPHKJ_00087 73231 4 Gut 0.57 protein_coding synonymous_variant LOW 537C>T Asp179Asp
M0198066 FOMKPHKJ_00087 73234 4 Gut 0.57 protein_coding synonymous_variant LOW 534C>T Asn178Asn
M0198067 FOMKPHKJ_00087 73298 4 Gut 0.57 protein_coding missense_variant MODERATE 470A>G His157Arg
M0198068 FOMKPHKJ_00087 73342 4 Gut 0.57 protein_coding synonymous_variant LOW 426T>A Thr142Thr
M0198069 FOMKPHKJ_00087 73376 4 Gut 0.57 protein_coding missense_variant MODERATE 392T>C Val131Ala
M0198070 FOMKPHKJ_00087 73380 4 Gut 0.57 protein_coding missense_variant MODERATE 388G>T Val130Leu
M0198071 FOMKPHKJ_00087 73387 4 Gut 0.57 protein_coding synonymous_variant LOW 381C>T Thr127Thr
M0198072 FOMKPHKJ_00087 73457 4 Gut 0.57 protein_coding missense_variant MODERATE 311A>G Gln104Arg
M0198073 FOMKPHKJ_00087 73498 4 Gut 0.57 protein_coding synonymous_variant LOW 270T>C Arg90Arg
M0198074 FOMKPHKJ_00087 73557 4 Gut 0.57 protein_coding missense_variant MODERATE 211G>A Val71Ile
M0198075 FOMKPHKJ_00087 73602 4 Gut 0.57 protein_coding missense_variant MODERATE 166T>G Ser56Ala
M0198076 FOMKPHKJ_00087 73603 4 Gut 0.57 protein_coding synonymous_variant LOW 165C>G Gly55Gly
M0198077 FOMKPHKJ_00087 73633 4 Gut 0.57 protein_coding synonymous_variant LOW 135T>A Ala45Ala
M0198078 FOMKPHKJ_00087 73670 4 Gut 0.57 protein_coding missense_variant MODERATE 98C>A Thr33Asn
M0198079 FOMKPHKJ_00087 73763 4 Gut 0.57 protein_coding missense_variant MODERATE 5T>C Leu2Ser
M0198080 FOMKPHKJ_00088 73771 4 Gut 0.57 protein_coding missense_variant MODERATE 2495C>T Ser832Leu
M0198081 FOMKPHKJ_00088 73772 4 Gut 0.57 protein_coding missense_variant MODERATE 2494T>A Ser832Thr
M0198082 FOMKPHKJ_00088 73827 4 Gut 0.57 protein_coding synonymous_variant LOW 2439C>T Val813Val
M0198083 FOMKPHKJ_00088 73857 4 Gut 0.57 protein_coding synonymous_variant LOW 2409G>A Thr803Thr
M0198084 FOMKPHKJ_00088 73902 4 Gut 0.57 protein_coding synonymous_variant LOW 2364T>C Gly788Gly
M0198085 FOMKPHKJ_00088 73911 4 Gut 0.57 protein_coding synonymous_variant LOW 2355T>G Val785Val
M0198086 FOMKPHKJ_00088 73920 4 Gut 0.57 protein_coding synonymous_variant LOW 2346T>C Ser782Ser
M0198087 FOMKPHKJ_00088 73922 4 Gut 0.57 protein_coding missense_variant MODERATE 2344T>A Ser782Thr
M0198088 FOMKPHKJ_00088 73923 4 Gut 0.57 protein_coding synonymous_variant LOW 2343T>C Asn781Asn
M0198089 FOMKPHKJ_00088 73962 4 Gut 0.57 protein_coding synonymous_variant LOW 2304C>A Thr768Thr
M0198090 FOMKPHKJ_00088 73971 4 Gut 0.57 protein_coding synonymous_variant LOW 2295T>A Pro765Pro
M0198091 FOMKPHKJ_00088 74013 4 Gut 0.57 protein_coding synonymous_variant LOW 2253G>A Gln751Gln
M0198092 FOMKPHKJ_00088 74016 4 Gut 0.57 protein_coding synonymous_variant LOW 2250A>G Arg750Arg
M0198093 FOMKPHKJ_00088 74018 4 Gut 0.57 protein_coding missense_variant MODERATE 2248A>G Arg750Gly
M0198094 FOMKPHKJ_00088 74085 4 Gut 0.57 protein_coding synonymous_variant LOW 2181G>A Glu727Glu
M0198095 FOMKPHKJ_00088 74097 4 Gut 0.57 protein_coding synonymous_variant LOW 2169C>T Ile723Ile
M0198096 FOMKPHKJ_00088 74128 4 Gut 0.57 protein_coding missense_variant MODERATE 2138G>A Gly713Asp
M0198097 FOMKPHKJ_00088 74184 4 Gut 0.57 protein_coding synonymous_variant LOW 2082T>C Thr694Thr
M0198098 FOMKPHKJ_00088 74228 4 Gut 0.57 protein_coding missense_variant MODERATE 2038G>A Gly680Ser
M0198099 FOMKPHKJ_00088 74283 4 Gut 0.57 protein_coding synonymous_variant LOW 1983T>C Gly661Gly
M0198100 FOMKPHKJ_00088 74290 4 Gut 0.57 protein_coding missense_variant MODERATE 1976G>A Gly659Asp
M0198101 FOMKPHKJ_00088 74313 4 Gut 0.57 protein_coding synonymous_variant LOW 1953A>G Leu651Leu
M0198102 FOMKPHKJ_00088 74325 4 Gut 0.57 protein_coding synonymous_variant LOW 1941T>G Thr647Thr
M0198103 FOMKPHKJ_00088 74373 4 Gut 0.57 protein_coding synonymous_variant LOW 1893T>C Phe631Phe
M0198104 FOMKPHKJ_00088 74754 4 Gut 0.57 protein_coding synonymous_variant LOW 1512T>G Leu504Leu
M0198105 FOMKPHKJ_00088 76238 3 Gut 0.43 protein_coding missense_variant MODERATE 28T>C Ser10Pro
M0198106 FOMKPHKJ_00089 76352 3 Gut 0.43 protein_coding synonymous_variant LOW 378A>G Val126Val
M0198107 FOMKPHKJ_00090 77298 3 Gut 0.43 protein_coding synonymous_variant LOW 117C>T Ser39Ser
M0198108 FOMKPHKJ_00085 77421 3 Gut 0.43 protein_coding upstream_gene_variant MODIFIER -4971G>C None
M0198109 FOMKPHKJ_00091 77554 3 Gut 0.43 protein_coding missense_variant MODERATE 523A>T Thr175Ser
M0198110 FOMKPHKJ_00091 77616 3 Gut 0.43 protein_coding missense_variant MODERATE 461C>A Thr154Lys
M0198111 FOMKPHKJ_00091 77627 3 Gut 0.43 protein_coding missense_variant MODERATE 450A>T Lys150Asn
M0198112 FOMKPHKJ_00091 77628 3 Gut 0.43 protein_coding missense_variant MODERATE 449A>G Lys150Arg
M0198113 FOMKPHKJ_00091 77631 3 Gut 0.43 protein_coding missense_variant MODERATE 446A>G Asp149Gly
M0198114 FOMKPHKJ_00091 77634 3 Gut 0.43 protein_coding missense_variant MODERATE 443A>G Asn148Ser
M0198115 FOMKPHKJ_00091 77655 3 Gut 0.43 protein_coding missense_variant MODERATE 422A>T His141Leu
M0198116 FOMKPHKJ_00091 77659 3 Gut 0.43 protein_coding missense_variant MODERATE 418A>G Asn140Asp
M0198117 FOMKPHKJ_00091 77729 3 Gut 0.43 protein_coding synonymous_variant LOW 348T>C Asp116Asp
M0198118 FOMKPHKJ_00091 77758 3 Gut 0.43 protein_coding missense_variant MODERATE 319A>G Thr107Ala
M0198119 FOMKPHKJ_00091 77769 3 Gut 0.43 protein_coding missense_variant MODERATE 308C>G Ala103Gly
M0198120 FOMKPHKJ_00091 77811 3 Gut 0.43 protein_coding missense_variant MODERATE 266C>A Thr89Lys
M0198121 FOMKPHKJ_00091 77819 3 Gut 0.43 protein_coding missense_variant MODERATE 258T>A Asp86Glu
M0198122 FOMKPHKJ_00087 78243 4 Gut 0.57 protein_coding upstream_gene_variant MODIFIER -4476C>T None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
FOMKPHKJ_00157 VFG001445 TraJ 98.5 1.2e-113 1 200 0.8772 0.995 Invasion unknown protein experiment
FOMKPHKJ_00129 VFG033809 Vat 92.3 0 1 1363 1.0 0.9993 Effector delivery system vacuolating autotransporter toxin prediction
FOMKPHKJ_00157 VFG001445 TraJ 98.5 9.3e-113 1 200 0.8772 0.995 Invasion unknown protein prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
FOMKPHKJ_00155 AHG18083.1|GH23 99.1 1.36e-155 1 215 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term