Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C1224
  Reference Plasmid   NZ_CP137058.1
  Reference Plasmid Size   107668
  Reference Plasmid GC Content   0.37
  Reference Plasmid Mobility Type   mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0114938 ECDBOJKI_00039 32042 4 Skin 0.31 protein_coding synonymous_variant LOW 309T>C Leu103Leu
M0114939 ECDBOJKI_00039 32099 4 Skin 0.31 protein_coding synonymous_variant LOW 252A>G Glu84Glu
M0114940 ECDBOJKI_00039 32105 4 Skin 0.31 protein_coding synonymous_variant LOW 246T>G Val82Val
M0114941 ECDBOJKI_00039 32163 4 Skin 0.31 protein_coding missense_variant MODERATE 188C>T Thr63Ile
M0114942 ECDBOJKI_00039 32192 4 Skin 0.31 protein_coding synonymous_variant LOW 159A>G Val53Val
M0114943 ECDBOJKI_00039 32321 3 Skin 0.23 protein_coding synonymous_variant LOW 30A>G Glu10Glu
M0114944 ECDBOJKI_00039 32370 5 Skin 0.38 protein_coding upstream_gene_variant MODIFIER -20G>T None
M0114945 ECDBOJKI_00040 32507 4 Skin 0.31 protein_coding missense_variant MODERATE 88G>A Ala30Thr
M0114946 ECDBOJKI_00040 32537 4 Skin 0.31 protein_coding missense_variant MODERATE 118A>G Thr40Ala
M0114947 ECDBOJKI_00040 32554 3 Skin 0.23 protein_coding synonymous_variant LOW 135T>C Ser45Ser
M0114948 ECDBOJKI_00040 32596 5 Skin 0.38 protein_coding synonymous_variant LOW 177T>A Pro59Pro
M0114949 ECDBOJKI_00040 32657 3 Skin 0.23 protein_coding missense_variant MODERATE 238G>A Gly80Ser
M0114950 ECDBOJKI_00040 32658 3 Skin 0.23 protein_coding missense_variant MODERATE 239G>C Gly80Ala
M0114951 ECDBOJKI_00040 32663 3 Skin 0.23 protein_coding missense_variant MODERATE 244A>G Asn82Asp
M0114952 ECDBOJKI_00040 32668 3 Skin 0.23 protein_coding synonymous_variant LOW 249G>A Ser83Ser
M0114953 ECDBOJKI_00040 32685 3 Skin 0.23 protein_coding missense_variant MODERATE 266C>T Thr89Ile
M0114954 ECDBOJKI_00040 32687 3 Skin 0.23 protein_coding missense_variant MODERATE 268G>A Val90Ile
M0114955 ECDBOJKI_00040 32689 3 Skin 0.23 protein_coding synonymous_variant LOW 270T>C Val90Val
M0114956 ECDBOJKI_00040 32691 3 Skin 0.23 protein_coding missense_variant MODERATE 272A>G Asn91Ser
M0114957 ECDBOJKI_00040 32731 3 Skin 0.23 protein_coding missense_variant MODERATE 312C>A Asn104Lys
M0114958 ECDBOJKI_00040 32792 4 Skin 0.31 protein_coding missense_variant MODERATE 373T>G Leu125Val
M0114959 ECDBOJKI_00040 32797 4 Skin 0.31 protein_coding synonymous_variant LOW 378C>T Asn126Asn
M0114960 ECDBOJKI_00040 32803 4 Skin 0.31 protein_coding synonymous_variant LOW 384A>G Lys128Lys
M0114961 ECDBOJKI_00040 32899 3 Skin 0.23 protein_coding synonymous_variant LOW 480A>C Gly160Gly
M0114962 ECDBOJKI_00040 32902 3 Skin 0.23 protein_coding synonymous_variant LOW 483A>T Ala161Ala
M0114963 ECDBOJKI_00040 32965 5 Skin 0.38 protein_coding synonymous_variant LOW 546A>G Leu182Leu
M0114964 ECDBOJKI_00040 33027 3 Skin 0.23 protein_coding missense_variant MODERATE 608A>G Asn203Ser
M0114965 ECDBOJKI_00040 33045 3 Skin 0.23 protein_coding missense_variant MODERATE 626C>T Thr209Ile
M0114966 ECDBOJKI_00040 33070 3 Skin 0.23 protein_coding synonymous_variant LOW 651G>A Leu217Leu
M0114967 ECDBOJKI_00040 33121 4 Skin 0.31 protein_coding synonymous_variant LOW 702T>A Ala234Ala
M0114968 ECDBOJKI_00040 33127 4 Skin 0.31 protein_coding synonymous_variant LOW 708G>T Gly236Gly
M0114969 ECDBOJKI_00040 33671 4 Skin 0.31 protein_coding missense_variant MODERATE 1252A>G Ile418Val
M0114970 ECDBOJKI_00042 34129 3 Skin 0.23 protein_coding synonymous_variant LOW 132C>T Phe44Phe
M0114971 ECDBOJKI_00042 34315 5 Skin 0.38 protein_coding synonymous_variant LOW 318C>T Leu106Leu
M0114972 ECDBOJKI_00042 35017 4 Skin 0.31 protein_coding synonymous_variant LOW 1020T>C Ser340Ser
M0114973 ECDBOJKI_00042 35104 3 Skin 0.23 protein_coding synonymous_variant LOW 1107G>A Leu369Leu
M0114974 ECDBOJKI_00042 35284 3 Skin 0.23 protein_coding synonymous_variant LOW 1287A>G Leu429Leu
M0114975 ECDBOJKI_00042 35305 4 Skin 0.31 protein_coding synonymous_variant LOW 1308T>A Ala436Ala
M0114976 ECDBOJKI_00042 35320 4 Skin 0.31 protein_coding synonymous_variant LOW 1323C>T Asp441Asp
M0114977 ECDBOJKI_00042 35335 4 Skin 0.31 protein_coding synonymous_variant LOW 1338C>T Phe446Phe
M0114978 ECDBOJKI_00042 35350 4 Skin 0.31 protein_coding synonymous_variant LOW 1353C>T Gly451Gly
M0114979 ECDBOJKI_00042 35386 4 Skin 0.31 protein_coding synonymous_variant LOW 1389A>G Val463Val
M0114980 ECDBOJKI_00042 35392 4 Skin 0.31 protein_coding synonymous_variant LOW 1395G>T Val465Val
M0114981 ECDBOJKI_00042 35407 4 Skin 0.31 protein_coding synonymous_variant LOW 1410T>A Pro470Pro
M0114982 ECDBOJKI_00042 35422 3 Skin 0.23 protein_coding synonymous_variant LOW 1425A>G Thr475Thr
M0114983 ECDBOJKI_00042 35452 4 Skin 0.31 protein_coding synonymous_variant LOW 1455G>A Gly485Gly
M0114984 ECDBOJKI_00042 35464 4 Skin 0.31 protein_coding synonymous_variant LOW 1467T>C His489His
M0114985 ECDBOJKI_00042 35647 4 Skin 0.31 protein_coding synonymous_variant LOW 1650A>G Gly550Gly
M0114986 ECDBOJKI_00042 35648 4 Skin 0.31 protein_coding synonymous_variant LOW 1651T>C Leu551Leu
M0114987 ECDBOJKI_00039 35864 4 Skin 0.31 protein_coding upstream_gene_variant MODIFIER -3514C>G None
M0114988 ECDBOJKI_00039 35865 4 Skin 0.31 protein_coding upstream_gene_variant MODIFIER -3515T>A None
M0114989 ECDBOJKI_00039 35866 4 Skin 0.31 protein_coding upstream_gene_variant MODIFIER -3516T>A None
M0114990 ECDBOJKI_00039 35867 4 Skin 0.31 protein_coding upstream_gene_variant MODIFIER -3517C>G None
M0114991 ECDBOJKI_00043 36000 4 Skin 0.31 protein_coding missense_variant MODERATE 262T>C Ser88Pro
M0114992 ECDBOJKI_00043 36223 4 Skin 0.31 protein_coding synonymous_variant LOW 39C>T Thr13Thr
M0114993 ECDBOJKI_00043 36242 4 Skin 0.31 protein_coding missense_variant MODERATE 20C>T Thr7Ile
M0114994 ECDBOJKI_00044 36370 3 Skin 0.23 protein_coding synonymous_variant LOW 3063C>G Gly1021Gly
M0114995 ECDBOJKI_00044 36390 4 Skin 0.31 protein_coding missense_variant MODERATE 3043A>G Thr1015Ala
M0114996 ECDBOJKI_00044 36499 3 Skin 0.23 protein_coding synonymous_variant LOW 2934G>A Gly978Gly
M0114997 ECDBOJKI_00044 36516 3 Skin 0.23 protein_coding missense_variant MODERATE 2917G>A Glu973Lys
M0114998 ECDBOJKI_00044 36527 4 Skin 0.31 protein_coding missense_variant MODERATE 2906G>A Arg969Gln
M0114999 ECDBOJKI_00044 36712 3 Skin 0.23 protein_coding synonymous_variant LOW 2721G>C Ala907Ala
M0115000 ECDBOJKI_00044 36919 4 Skin 0.31 protein_coding missense_variant MODERATE 2514T>A His838Gln
M0115001 ECDBOJKI_00044 36963 3 Skin 0.23 protein_coding missense_variant MODERATE 2470G>A Val824Ile
M0115002 ECDBOJKI_00044 37063 3 Skin 0.23 protein_coding synonymous_variant LOW 2370A>T Thr790Thr
M0115003 ECDBOJKI_00044 37380 3 Skin 0.23 protein_coding missense_variant MODERATE 2053A>G Ile685Val
M0115004 ECDBOJKI_00044 37597 3 Skin 0.23 protein_coding synonymous_variant LOW 1836T>A Ala612Ala
M0115005 ECDBOJKI_00044 37954 3 Skin 0.23 protein_coding synonymous_variant LOW 1479T>C Ser493Ser
M0115006 ECDBOJKI_00044 38215 3 Skin 0.23 protein_coding synonymous_variant LOW 1218T>C Ala406Ala
M0115007 ECDBOJKI_00044 38221 3 Skin 0.23 protein_coding synonymous_variant LOW 1212T>C Val404Val






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group
ECDBOJKI_00051 Cadmium (Cd), Zinc (Zn) 81.9 3.3e-107 1 226 0.9956 0.9869 prediction
ECDBOJKI_00053 Copper (Cu) 76.4 0 5 784 0.9949 0.9874 prediction






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
ECDBOJKI_00048 PHI:10396 copB (ABUW_3320) 83.3 1.8e-146 1 306 1.0000 1.0000 moths nosocomial infection autotransporter domain-containing protein reduced virulence
ECDBOJKI_00049 PHI:10392 pcoA (ABUW_3228) 70.5 1.5e-264 1 627 1.0000 1.0000 moths nosocomial infection copper resistance protein A reduced virulence
ECDBOJKI_00051 PHI:10397 cusR (ABUW_3323) 96.9 4.1e-124 1 227 1.0000 1.0000 moths nosocomial infection DNA-binding response regulator reduced virulence
ECDBOJKI_00052 PHI:10394 cusS (ABUW_3324) 91.2 7.3e-237 1 456 0.9978 0.9978 moths nosocomial infection sensor kinase reduced virulence
ECDBOJKI_00054 PHI:10398 copC (ABUW_3326) 92.1 1.6e-61 1 126 1.0000 1.0000 moths nosocomial infection copper resistance protein reduced virulence
ECDBOJKI_00055 PHI:10395 copD (ABUW_3327) 89.8 1.6e-145 36 328 0.8933 1.0000 moths nosocomial infection copper resistance D reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
ECDBOJKI_00055 9.B.62.1.6 89.8 3.6e-145 36 328 0.8933 1.0000 9 Incompletely Characterized Transport Systems 9.B Putative transport proteins 9.B.62 The Copper Resistance (CopD) Family