Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C1230
  Reference Plasmid   NZ_CP138657.1
  Reference Plasmid Size   82106
  Reference Plasmid GC Content   0.35
  Reference Plasmid Mobility Type   mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0200492 KELNOENG_00069 63860 7 Gut 0.44 protein_coding synonymous_variant LOW 249A>G Lys83Lys
M0200493 KELNOENG_00063 59375 5 Gut 0.31 protein_coding missense_variant MODERATE 1321A>G Thr441Ala
M0200494 KELNOENG_00058 52543 7 Gut 0.44 protein_coding upstream_gene_variant MODIFIER -2284A>G None
M0200495 KELNOENG_00060 56151 3 Gut 0.19 protein_coding synonymous_variant LOW 357C>T Asp119Asp
M0200496 KELNOENG_00080 73460 3 Gut 0.19 protein_coding upstream_gene_variant MODIFIER -42T>C None
M0200497 KELNOENG_00080 73487 3 Gut 0.19 protein_coding upstream_gene_variant MODIFIER -15C>A None
M0200498 KELNOENG_00080 73529 3 Gut 0.19 protein_coding missense_variant MODERATE 28C>T Pro10Ser
M0200499 KELNOENG_00080 73547 3 Gut 0.19 protein_coding synonymous_variant LOW 46C>A Arg16Arg
M0200500 KELNOENG_00080 73598 3 Gut 0.19 protein_coding missense_variant MODERATE 97A>C Lys33Gln
M0200501 KELNOENG_00080 73599 3 Gut 0.19 protein_coding missense_variant MODERATE 98A>G Lys33Arg
M0200502 KELNOENG_00080 73603 3 Gut 0.19 protein_coding synonymous_variant LOW 102C>T Ala34Ala
M0200503 KELNOENG_00080 73618 3 Gut 0.19 protein_coding synonymous_variant LOW 117A>C Ile39Ile
M0200504 KELNOENG_00080 73636 3 Gut 0.19 protein_coding synonymous_variant LOW 135G>T Thr45Thr
M0200505 KELNOENG_00080 73657 3 Gut 0.19 protein_coding synonymous_variant LOW 156A>G Arg52Arg
M0200506 KELNOENG_00080 73678 3 Gut 0.19 protein_coding synonymous_variant LOW 177A>T Leu59Leu
M0200507 KELNOENG_00080 73691 3 Gut 0.19 protein_coding missense_variant MODERATE 190C>A Pro64Thr
M0200508 KELNOENG_00080 73727 3 Gut 0.19 protein_coding synonymous_variant LOW 226C>T Leu76Leu
M0200509 KELNOENG_00080 73729 3 Gut 0.19 protein_coding synonymous_variant LOW 228A>G Leu76Leu
M0200510 KELNOENG_00080 73741 3 Gut 0.19 protein_coding synonymous_variant LOW 240C>T Ile80Ile
M0200511 KELNOENG_00080 73756 3 Gut 0.19 protein_coding synonymous_variant LOW 255G>A Ser85Ser






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
KELNOENG_00010 VFG002164 AS 95.1 0 1 1305 1.0 1 Adherence aggregation substance PrgB/Asc10 experiment
KELNOENG_00073 VFG002162 BSH 73.8 1.9e-147 1 324 1.0 0.9969 Stress survival bile salt hydrolase experiment
KELNOENG_00010 VFG002164 AS 95.1 0 1 1305 1.0 1 Adherence aggregation substance PrgB/Asc10 prediction
KELNOENG_00073 VFG019385 BSH 74.4 3.3e-148 1 324 1.0 0.9969 Stress survival bile salt hydrolase prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
KELNOENG_00031 PHI:2999 EF0026 98.1 3.3e-91 1 162 1.0000 1.0000 moths nosocomial infection None increased virulence (hypervirulence)






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
KELNOENG_00016 QGI54475.1|GH73 99.5 1.4e-280 1 383 1 1
KELNOENG_00048 CCO10203.2|GH1 87.9 4.75e-247 14 401 0.9628 0.9949
KELNOENG_00064 QZK92746.1|GH1 100 0 1 468 1 1
KELNOENG_00077 CCO10203.2|GH1 87.9 4.75e-247 14 401 0.9628 0.9949





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
KELNOENG_00063 4.A.3.1.1 71.9 8.8e-240 1 568 1.0000 0.9965 4 Group Translocators 4.A Phosphotransfer-driven Group Translocators (PTS) 4.A.3 The PTS Lactose-N,N'-Diacetylchitobiose-β-glucoside (Lac) Family