Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C1315
  Reference Plasmid   NZ_CP152304.1
  Reference Plasmid Size   6774
  Reference Plasmid GC Content   0.51
  Reference Plasmid Mobility Type   mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0206303 DEBIHKEN_00001 278 3 Gut 0.75 protein_coding synonymous_variant LOW 174A>G Thr58Thr
M0206304 DEBIHKEN_00001 320 3 Gut 0.75 protein_coding synonymous_variant LOW 216T>G Pro72Pro
M0206305 DEBIHKEN_00001 872 3 Gut 0.75 protein_coding synonymous_variant LOW 768T>C Gly256Gly
M0206306 DEBIHKEN_00003 1070 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -598C>T None
M0206307 DEBIHKEN_00003 1213 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -455T>C None
M0206308 DEBIHKEN_00003 1216 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -452C>A None
M0206309 DEBIHKEN_00003 1217 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -451T>G None
M0206310 DEBIHKEN_00003 1218 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -450C>T None
M0206311 DEBIHKEN_00003 1219 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -449C>G None
M0206312 DEBIHKEN_00002 1225 3 Gut 0.75 protein_coding stop_lost&splice_region_variant HIGH 265T>C Ter89Glnext*?
M0206313 DEBIHKEN_00002 1235 3 Gut 0.75 protein_coding synonymous_variant LOW 255T>C Pro85Pro
M0206314 DEBIHKEN_00002 1242 3 Gut 0.75 protein_coding missense_variant MODERATE 248A>G Lys83Arg
M0206315 DEBIHKEN_00002 1251 3 Gut 0.75 protein_coding missense_variant MODERATE 239A>T Lys80Met
M0206316 DEBIHKEN_00002 1279 3 Gut 0.75 protein_coding missense_variant MODERATE 211A>C Ile71Leu
M0206317 DEBIHKEN_00002 1280 3 Gut 0.75 protein_coding synonymous_variant LOW 210G>A Arg70Arg
M0206318 DEBIHKEN_00002 1292 3 Gut 0.75 protein_coding synonymous_variant LOW 198C>G Ala66Ala
M0206319 DEBIHKEN_00002 1319 3 Gut 0.75 protein_coding synonymous_variant LOW 171C>T Phe57Phe
M0206320 DEBIHKEN_00002 1349 3 Gut 0.75 protein_coding synonymous_variant LOW 141G>A Glu47Glu
M0206321 DEBIHKEN_00002 1492 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -3A>G None
M0206322 DEBIHKEN_00002 1501 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -12T>C None
M0206323 DEBIHKEN_00003 3221 3 Gut 0.75 protein_coding missense_variant MODERATE 1554G>A Met518Ile
M0206324 DEBIHKEN_00003 3375 3 Gut 0.75 protein_coding missense_variant MODERATE 1708G>A Asp570Asn
M0206325 DEBIHKEN_00003 3404 3 Gut 0.75 protein_coding synonymous_variant LOW 1737G>A Leu579Leu
M0206326 DEBIHKEN_00003 3438 3 Gut 0.75 protein_coding missense_variant MODERATE 1771A>G Thr591Ala
M0206327 DEBIHKEN_00003 3509 3 Gut 0.75 protein_coding synonymous_variant LOW 1842A>G Leu614Leu
M0206328 DEBIHKEN_00003 3559 3 Gut 0.75 protein_coding missense_variant MODERATE 1892G>T Gly631Val
M0206329 DEBIHKEN_00003 3608 3 Gut 0.75 protein_coding missense_variant MODERATE 1941A>G Ile647Met
M0206330 DEBIHKEN_00004 4290 3 Gut 0.75 protein_coding synonymous_variant LOW 171T>G Gly57Gly
M0206331 DEBIHKEN_00004 4839 3 Gut 0.75 protein_coding synonymous_variant LOW 720A>G Ala240Ala
M0206332 DEBIHKEN_00004 5396 3 Gut 0.75 protein_coding missense_variant MODERATE 1277T>G Ile426Ser
M0206333 DEBIHKEN_00003 6697 3 Gut 0.75 protein_coding downstream_gene_variant MODIFIER *2810T>G None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
DEBIHKEN_00004 1.C.1.4.1 95.2 4.7e-230 1 459 0.8255 0.7904 1 Channels/Pores 1.C Pore-Forming Toxins (Proteins and Peptides) 1.C.1 The Channel-forming Colicin (Colicin) Family
DEBIHKEN_00006 1.A.73.1.3 91.5 8.6e-16 1 47 1.0000 1.0000 1 Channels/Pores 1.A α-Type Channels 1.A.73 The Colicin Lysis Protein (CLP) Family