Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C1334
  Reference Plasmid   NZ_KU932021.1
  Reference Plasmid Size   91885
  Reference Plasmid GC Content   0.53
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0206710 OJNKBEKM_00031 31636 3 Gut 0.30 protein_coding upstream_gene_variant MODIFIER -576T>A None
M0206711 OJNKBEKM_00032 32778 3 Gut 0.30 protein_coding missense_variant MODERATE 34A>G Thr12Ala
M0206712 OJNKBEKM_00035 33789 3 Gut 0.30 protein_coding missense_variant MODERATE 422C>T Ala141Val
M0206713 OJNKBEKM_00035 33893 3 Gut 0.30 protein_coding synonymous_variant LOW 318T>C Val106Val
M0206714 OJNKBEKM_00036 34327 3 Gut 0.30 protein_coding synonymous_variant LOW 15A>C Ile5Ile
M0206715 OJNKBEKM_00036 34354 3 Gut 0.30 protein_coding synonymous_variant LOW 42C>T Ala14Ala
M0206716 OJNKBEKM_00030 35714 3 Gut 0.30 protein_coding upstream_gene_variant MODIFIER -3832G>T None
M0206717 OJNKBEKM_00038 35885 3 Gut 0.30 protein_coding missense_variant MODERATE 64C>A Leu22Ile
M0206718 OJNKBEKM_00038 35886 3 Gut 0.30 protein_coding synonymous_variant LOW 63T>A Ala21Ala
M0206719 OJNKBEKM_00046 45216 3 Gut 0.30 protein_coding synonymous_variant LOW 372G>A Glu124Glu
M0206720 OJNKBEKM_00055 56116 3 Gut 0.30 protein_coding missense_variant MODERATE 97A>G Thr33Ala
M0206721 OJNKBEKM_00056 56372 3 Gut 0.30 protein_coding missense_variant MODERATE 113T>C Leu38Pro
M0206722 OJNKBEKM_00060 58521 3 Gut 0.30 protein_coding synonymous_variant LOW 309G>T Thr103Thr
M0206723 OJNKBEKM_00060 58527 3 Gut 0.30 protein_coding synonymous_variant LOW 303C>T Ile101Ile
M0206724 OJNKBEKM_00060 58530 3 Gut 0.30 protein_coding synonymous_variant LOW 300C>T Ala100Ala






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
OJNKBEKM_00043 ARO:3000412 100 4.11e-186 1 271 1.0000 1.0000 sulfonamide antibiotic sulfonamide resistant sul antibiotic target replacement
OJNKBEKM_00044 ARO:3002639 99.6 5.98e-202 1 267 1.0000 1.0000 aminoglycoside antibiotic APH(3'') antibiotic inactivation
OJNKBEKM_00045 ARO:3000873 100 5.12e-208 1 286 1.0000 1.0000 monobactam TEM beta-lactamase antibiotic inactivation
OJNKBEKM_00048 ARO:3002660 99.6 5.98e-177 15 257 0.9455 0.8741 aminoglycoside antibiotic APH(6) antibiotic inactivation






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
OJNKBEKM_00004 SPE00673.1|GH23 100 1.33e-115 1 161 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
OJNKBEKM_00010 3.A.7.10.1 77.9 6.6e-124 1 271 0.9748 2.8021 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
OJNKBEKM_00011 3.A.7.10.1 77.8 1.7e-175 1 379 0.9921 3.9479 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
OJNKBEKM_00014 3.A.7.10.1 73.3 1.9e-40 1 116 1.0000 1.1979 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
OJNKBEKM_00016 3.A.7.10.1 74.3 1.2e-137 1 324 1.0000 3.4063 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
OJNKBEKM_00030 1.E.53.1.10 100 2e-18 8 50 0.8600 1.0000 1 Channels/Pores 1.E Holins 1.E.53 The Toxic Hok/Gef Protein (Hok/Gef) Family
OJNKBEKM_00039 3.A.7.10.1 76.1 1.1e-189 1 401 0.9282 4.1771 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
OJNKBEKM_00041 3.A.7.10.1 79.6 0 1 724 0.9439 7.5417 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family