Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C1409
  Reference Plasmid   NZ_OP378608.1
  Reference Plasmid Size   94306
  Reference Plasmid GC Content   0.48
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0209369 ENNDKDHL_00065 66727 3 Gut 0.43 protein_coding synonymous_variant LOW 108A>C Ala36Ala
M0209370 ENNDKDHL_00065 66967 3 Gut 0.43 protein_coding synonymous_variant LOW 348A>G Ser116Ser
M0209371 ENNDKDHL_00065 67090 3 Gut 0.43 protein_coding synonymous_variant LOW 471T>G Leu157Leu
M0209372 ENNDKDHL_00067 68318 3 Gut 0.43 protein_coding synonymous_variant LOW 990A>T Arg330Arg
M0209373 ENNDKDHL_00067 68333 3 Gut 0.43 protein_coding synonymous_variant LOW 975C>T Phe325Phe
M0209374 ENNDKDHL_00067 68495 3 Gut 0.43 protein_coding synonymous_variant LOW 813T>C Phe271Phe
M0209375 ENNDKDHL_00067 68754 3 Gut 0.43 protein_coding missense_variant MODERATE 554T>C Met185Thr
M0209376 ENNDKDHL_00067 69004 3 Gut 0.43 protein_coding missense_variant MODERATE 304A>G Asn102Asp
M0209377 ENNDKDHL_00067 69083 3 Gut 0.43 protein_coding synonymous_variant LOW 225A>T Thr75Thr
M0209378 ENNDKDHL_00067 69088 3 Gut 0.43 protein_coding missense_variant MODERATE 220A>C Met74Leu
M0209379 ENNDKDHL_00068 69381 3 Gut 0.43 protein_coding synonymous_variant LOW 714A>G Glu238Glu
M0209380 ENNDKDHL_00068 69618 3 Gut 0.43 protein_coding synonymous_variant LOW 477C>A Ile159Ile
M0209381 ENNDKDHL_00068 69792 3 Gut 0.43 protein_coding synonymous_variant LOW 303A>G Pro101Pro
M0209382 ENNDKDHL_00068 69966 3 Gut 0.43 protein_coding synonymous_variant LOW 129A>G Ala43Ala
M0209383 ENNDKDHL_00069 70267 3 Gut 0.43 protein_coding synonymous_variant LOW 543G>A Leu181Leu
M0209384 ENNDKDHL_00069 70446 3 Gut 0.43 protein_coding missense_variant MODERATE 364G>A Asp122Asn
M0209385 ENNDKDHL_00069 70804 3 Gut 0.43 protein_coding synonymous_variant LOW 6A>T Ala2Ala
M0209386 ENNDKDHL_00070 70873 3 Gut 0.43 protein_coding synonymous_variant LOW 1158T>C Thr386Thr
M0209387 ENNDKDHL_00070 70972 3 Gut 0.43 protein_coding synonymous_variant LOW 1059C>T Ala353Ala
M0209388 ENNDKDHL_00070 70996 3 Gut 0.43 protein_coding synonymous_variant LOW 1035C>A Ala345Ala
M0209389 ENNDKDHL_00070 71182 3 Gut 0.43 protein_coding synonymous_variant LOW 849A>G Ser283Ser
M0209390 ENNDKDHL_00070 71323 3 Gut 0.43 protein_coding synonymous_variant LOW 708G>T Val236Val
M0209391 ENNDKDHL_00070 71335 3 Gut 0.43 protein_coding synonymous_variant LOW 696C>T Ala232Ala
M0209392 ENNDKDHL_00070 71338 3 Gut 0.43 protein_coding synonymous_variant LOW 693A>G Val231Val
M0209393 ENNDKDHL_00070 71365 3 Gut 0.43 protein_coding synonymous_variant LOW 666G>A Lys222Lys
M0209394 ENNDKDHL_00070 71572 3 Gut 0.43 protein_coding synonymous_variant LOW 459T>C His153His
M0209395 ENNDKDHL_00045 48489 3 Gut 0.43 protein_coding synonymous_variant LOW 1203T>A Arg401Arg
M0209396 ENNDKDHL_00045 48649 3 Gut 0.43 protein_coding missense_variant MODERATE 1043T>C Val348Ala
M0209397 ENNDKDHL_00045 48663 3 Gut 0.43 protein_coding synonymous_variant LOW 1029T>C Phe343Phe
M0209398 ENNDKDHL_00045 48709 3 Gut 0.43 protein_coding missense_variant MODERATE 983A>T Tyr328Phe
M0209399 ENNDKDHL_00045 48831 3 Gut 0.43 protein_coding synonymous_variant LOW 861A>T Ala287Ala
M0209400 ENNDKDHL_00045 48833 3 Gut 0.43 protein_coding missense_variant MODERATE 859G>T Ala287Ser
M0209401 ENNDKDHL_00045 48840 3 Gut 0.43 protein_coding missense_variant MODERATE 852A>T Lys284Asn
M0209402 ENNDKDHL_00045 48849 3 Gut 0.43 protein_coding synonymous_variant LOW 843A>G Ala281Ala
M0209403 ENNDKDHL_00045 48858 3 Gut 0.43 protein_coding synonymous_variant LOW 834T>G Ala278Ala
M0209404 ENNDKDHL_00045 48876 3 Gut 0.43 protein_coding synonymous_variant LOW 816C>G Gly272Gly
M0209405 ENNDKDHL_00045 48885 3 Gut 0.43 protein_coding synonymous_variant LOW 807T>C Leu269Leu
M0209406 ENNDKDHL_00045 48915 3 Gut 0.43 protein_coding synonymous_variant LOW 777T>C Asn259Asn
M0209407 ENNDKDHL_00045 49581 3 Gut 0.43 protein_coding synonymous_variant LOW 111A>G Leu37Leu
M0209408 ENNDKDHL_00046 49938 3 Gut 0.43 protein_coding synonymous_variant LOW 1521C>T Ala507Ala
M0209409 ENNDKDHL_00046 50751 3 Gut 0.43 protein_coding synonymous_variant LOW 708T>C Asn236Asn
M0209410 ENNDKDHL_00046 50859 3 Gut 0.43 protein_coding synonymous_variant LOW 600C>T Arg200Arg
M0209411 ENNDKDHL_00047 51895 3 Gut 0.43 protein_coding missense_variant MODERATE 791G>C Gly264Ala
M0209412 ENNDKDHL_00047 52398 3 Gut 0.43 protein_coding synonymous_variant LOW 288C>T Val96Val
M0209413 ENNDKDHL_00048 52846 3 Gut 0.43 protein_coding synonymous_variant LOW 1053T>C Arg351Arg
M0209414 ENNDKDHL_00048 52882 3 Gut 0.43 protein_coding synonymous_variant LOW 1017C>T Leu339Leu
M0209415 ENNDKDHL_00048 52915 3 Gut 0.43 protein_coding synonymous_variant LOW 984T>C Ser328Ser
M0209416 ENNDKDHL_00046 55080 3 Gut 0.43 protein_coding upstream_gene_variant MODIFIER -3622T>C None
M0209417 ENNDKDHL_00046 56302 3 Gut 0.43 protein_coding upstream_gene_variant MODIFIER -4844C>T None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
ENNDKDHL_00006 AYL89119.1|GH24 97 2.39999996078331e-317 1753 2255 0.2231 0.9031
ENNDKDHL_00009 AJS09995.1|GH24 100 4.67e-139 1 185 1 1
ENNDKDHL_00030 SPE01079.1|GH23 100 0 1 1130 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
ENNDKDHL_00019 1.E.4.1.1 100 1.3e-54 1 109 1.0000 1.0000 1 Channels/Pores 1.E Holins 1.E.4 The LydA Holin (LydA Holin) Family
ENNDKDHL_00041 1.E.53.1.4 73.1 9.4e-19 1 67 0.9571 0.9571 1 Channels/Pores 1.E Holins 1.E.53 The Toxic Hok/Gef Protein (Hok/Gef) Family