Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C1449
  Reference Plasmid   NZ_OZ042071.1
  Reference Plasmid Size   73787
  Reference Plasmid GC Content   0.53
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0211013 AECGAFOA_00071 61131 3 Gut 0.33 protein_coding synonymous_variant LOW 2175T>A Ser725Ser
M0211014 AECGAFOA_00073 62482 3 Gut 0.33 protein_coding upstream_gene_variant MODIFIER -108G>A None
M0211015 AECGAFOA_00074 63659 4 Gut 0.44 protein_coding synonymous_variant LOW 189T>C Val63Val
M0211016 AECGAFOA_00074 64454 4 Gut 0.44 protein_coding synonymous_variant LOW 984G>A Leu328Leu
M0211017 AECGAFOA_00074 65337 5 Gut 0.56 protein_coding missense_variant MODERATE 1867T>C Ser623Pro
M0211018 AECGAFOA_00075 66028 5 Gut 0.56 protein_coding synonymous_variant LOW 327A>G Val109Val
M0211019 AECGAFOA_00075 67527 4 Gut 0.44 protein_coding missense_variant MODERATE 1826C>T Ala609Val
M0211020 AECGAFOA_00075 70703 5 Gut 0.56 protein_coding missense_variant MODERATE 5002A>G Met1668Val
M0211021 AECGAFOA_00077 71590 5 Gut 0.56 protein_coding missense_variant MODERATE 8T>A Leu3Gln
M0211022 AECGAFOA_00077 71726 5 Gut 0.56 protein_coding synonymous_variant LOW 144T>C Pro48Pro
M0211023 AECGAFOA_00077 72056 5 Gut 0.56 protein_coding synonymous_variant LOW 474T>C Ala158Ala
M0211024 AECGAFOA_00078 72534 3 Gut 0.33 protein_coding missense_variant MODERATE 29G>A Ser10Asn
M0211025 AECGAFOA_00039 34217 3 Gut 0.33 protein_coding synonymous_variant LOW 214T>C Leu72Leu
M0211026 AECGAFOA_00041 35080 4 Gut 0.44 protein_coding synonymous_variant LOW 198T>C Ser66Ser
M0211027 AECGAFOA_00041 35086 3 Gut 0.33 protein_coding missense_variant MODERATE 204G>T Lys68Asn
M0211028 AECGAFOA_00041 36475 3 Gut 0.33 protein_coding synonymous_variant LOW 1593T>C Thr531Thr
M0211029 AECGAFOA_00041 36562 3 Gut 0.33 protein_coding synonymous_variant LOW 1680T>G Leu560Leu
M0211030 AECGAFOA_00041 36565 3 Gut 0.33 protein_coding synonymous_variant LOW 1683C>T Asp561Asp
M0211031 AECGAFOA_00042 37072 4 Gut 0.44 protein_coding synonymous_variant LOW 168T>C Phe56Phe
M0211032 AECGAFOA_00042 37075 4 Gut 0.44 protein_coding synonymous_variant LOW 171C>G Gly57Gly
M0211033 AECGAFOA_00042 37078 4 Gut 0.44 protein_coding synonymous_variant LOW 174A>C Gly58Gly
M0211034 AECGAFOA_00042 37240 4 Gut 0.44 protein_coding synonymous_variant LOW 336C>T Ala112Ala
M0211035 AECGAFOA_00042 37294 4 Gut 0.44 protein_coding synonymous_variant LOW 390C>T Val130Val
M0211036 AECGAFOA_00042 37296 4 Gut 0.44 protein_coding missense_variant MODERATE 392G>A Ser131Asn
M0211037 AECGAFOA_00042 37297 4 Gut 0.44 protein_coding synonymous_variant LOW 393T>C Ser131Ser
M0211038 AECGAFOA_00042 37306 4 Gut 0.44 protein_coding synonymous_variant LOW 402C>T Ile134Ile
M0211039 AECGAFOA_00043 37340 4 Gut 0.44 protein_coding missense_variant MODERATE 5G>A Ser2Asn
M0211040 AECGAFOA_00043 37365 4 Gut 0.44 protein_coding synonymous_variant LOW 30C>T Pro10Pro
M0211041 AECGAFOA_00043 37394 4 Gut 0.44 protein_coding missense_variant MODERATE 59A>G Gln20Arg
M0211042 AECGAFOA_00043 37745 4 Gut 0.44 protein_coding missense_variant MODERATE 410G>A Gly137Glu
M0211043 AECGAFOA_00043 37821 4 Gut 0.44 protein_coding synonymous_variant LOW 486C>T Val162Val
M0211044 AECGAFOA_00044 38150 3 Gut 0.33 protein_coding synonymous_variant LOW 99A>G Pro33Pro






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
AECGAFOA_00024 VFG041000 AAI/SCI-II T6SS 90.3 1.7e-78 1 155 1.0 0.8245 Effector delivery system hypothetical protein prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
AECGAFOA_00006 ARO:3000316 100 1e-217 1 301 1.0000 1.0000 macrolide antibiotic macrolide phosphotransferase (MPH) antibiotic inactivation
AECGAFOA_00007 ARO:3003839 100 4.74e-272 1 412 1.0000 1.0000 macrolide antibiotic macrolide phosphotransferase (MPH) antibiotic inactivation
AECGAFOA_00012 ARO:3000873 100 5.12e-208 1 286 1.0000 1.0000 monobactam TEM beta-lactamase antibiotic inactivation






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
AECGAFOA_00025 PHI:3113 stbA 97.9 2.8e-182 1 326 1.0000 1.0000 rodents neonatal meningitis stability protein for the plasmid reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term