Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C3411
  Reference Plasmid   SRR1950730_bin.2__k91_9090
  Reference Plasmid Size   34229
  Reference Plasmid GC Content   0.32
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0116918 HBBJMNAH_00001 779 3 Skin 0.30 protein_coding missense_variant MODERATE 610G>A Ala204Thr
M0116919 HBBJMNAH_00001 808 3 Skin 0.30 protein_coding stop_lost&splice_region_variant HIGH 639A>G Ter213Trpext*?
M0116920 HBBJMNAH_00002 875 3 Skin 0.30 protein_coding missense_variant MODERATE 34T>G Phe12Val
M0116921 HBBJMNAH_00002 926 3 Skin 0.30 protein_coding missense_variant MODERATE 85A>G Ile29Val
M0116922 HBBJMNAH_00002 937 3 Skin 0.30 protein_coding synonymous_variant LOW 96G>A Val32Val
M0116923 HBBJMNAH_00002 1067 3 Skin 0.30 protein_coding missense_variant MODERATE 226G>T Ala76Ser
M0116924 HBBJMNAH_00002 1095 3 Skin 0.30 protein_coding missense_variant MODERATE 254A>G His85Arg
M0116925 HBBJMNAH_00002 1127 3 Skin 0.30 protein_coding missense_variant MODERATE 286A>G Ile96Val
M0116926 HBBJMNAH_00002 1174 3 Skin 0.30 protein_coding synonymous_variant LOW 333G>A Pro111Pro
M0116927 HBBJMNAH_00003 1407 3 Skin 0.30 protein_coding synonymous_variant LOW 216A>T Val72Val
M0116928 HBBJMNAH_00003 1602 3 Skin 0.30 protein_coding synonymous_variant LOW 411T>C Asp137Asp
M0116929 HBBJMNAH_00004 1840 3 Skin 0.30 protein_coding upstream_gene_variant MODIFIER -131T>G None
M0116930 HBBJMNAH_00008 5911 3 Skin 0.30 protein_coding synonymous_variant LOW 522A>G Gly174Gly
M0116931 HBBJMNAH_00008 5912 3 Skin 0.30 protein_coding missense_variant MODERATE 523G>T Ala175Ser
M0116932 HBBJMNAH_00008 6100 3 Skin 0.30 protein_coding missense_variant MODERATE 711A>T Lys237Asn
M0116933 HBBJMNAH_00009 6253 3 Skin 0.30 protein_coding missense_variant MODERATE 13A>G Asn5Asp
M0116934 HBBJMNAH_00009 6427 3 Skin 0.30 protein_coding stop_lost&splice_region_variant HIGH 187T>C Ter63Glnext*?
M0116935 HBBJMNAH_00007 6489 3 Skin 0.30 protein_coding upstream_gene_variant MODIFIER -1426T>A None
M0116936 HBBJMNAH_00010 6688 3 Skin 0.30 protein_coding missense_variant MODERATE 175A>C Ile59Leu
M0116937 HBBJMNAH_00010 6949 3 Skin 0.30 protein_coding synonymous_variant LOW 436A>C Arg146Arg
M0116938 HBBJMNAH_00010 6951 3 Skin 0.30 protein_coding missense_variant MODERATE 438A>T Arg146Ser
M0116939 HBBJMNAH_00010 6954 3 Skin 0.30 protein_coding synonymous_variant LOW 441G>A Lys147Lys
M0116940 HBBJMNAH_00010 6957 3 Skin 0.30 protein_coding synonymous_variant LOW 444T>G Thr148Thr
M0116941 HBBJMNAH_00010 6978 3 Skin 0.30 protein_coding synonymous_variant LOW 465A>C Val155Val
M0116942 HBBJMNAH_00010 7441 3 Skin 0.30 protein_coding missense_variant MODERATE 928G>T Asp310Tyr
M0116943 HBBJMNAH_00011 8051 3 Skin 0.30 protein_coding missense_variant MODERATE 568A>T Thr190Ser






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
HBBJMNAH_00020 PHI:6190 Mqo 80.7 1.8e-241 1 492 1.0000 1.0000 rodents skin infection; food poisoning; respiratory disease malate-quinone oxidoreductases reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
HBBJMNAH_00015 AXV41634.1|GT2 99.7 0 1 720 1 1
HBBJMNAH_00019 QKI07436.1|GT4 99.5 3.53e-273 1 381 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
HBBJMNAH_00030 2.A.1.2.100 72.9 7.8e-150 1 390 0.9726 0.9726 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.1 The Major Facilitator Superfamily (MFS)